Pig lactate dehydrogenase(LDH) ELISA Kit

Code CSB-EZ013482PI
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lactate dehydrogenase(LDH)
Abbreviation LDH
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 μU/mL-8000 μU/mL
Sensitivity 62.5 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of pig LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:100Average %84
Range %80-92
1:200Average %94
Range %90-100
1:400Average %98
Range %94-102
1:800Average %104
Range %100-113
The recovery of pig LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9790-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
80000.100 0.102 0.101
40000.213 0.206 0.210
20000.449 0.454 0.452
10000.756 0.719 0.738
5001.356 1.324 1.340
2501.674 1.710 1.692
1251.935 1.979 1.957
02.349 2.297 2.323
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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