Pig lipoprotein-associated phospholipase A2 (Lp-PLA2) ELISA Kit

Instructions
Code CSB-E15789p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lipoprotein-associated phospholipase A2 (Lp-PLA2)
Abbreviation Lp-PLA2
Uniprot No. Q4Z8N7
Species Sus scrofa (Pig)
Sample Types serum, plasma
Detection Range 46.88 ng/mL-3000ng/mL
Sensitivity 11.7 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 88-100
1:2 Average % 95
Range % 90-100
1:4 Average % 102
Range % 96-106
1:8 Average % 104
Range % 97-111
Recovery
The recovery of pig Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 96-105
EDTA plasma (n=4) 99 92-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
3000 2.301 2.222 2.262 2.082
1500 1.926 1.877 1.902 1.722
750 1.391 1.324 1.358 1.178
375 0.797 0.765 0.781 0.601
187.5 0.479 0.471 0.475 0.295
93.75 0.311 0.303 0.307 0.127
46.88 0.248 0.240 0.244 0.064
0 0.182 0.178 0.180  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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