Pig lipoprotein lipase (LPL) ELISA kit

Instructions
Code CSB-E17697p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lipoprotein lipase
Alternative Names LPLLipoprotein lipase ELISA Kit; LPL ELISA Kit; EC 3.1.1.34 ELISA Kit
Abbreviation LPL
Uniprot No. P49923
Species Sus scrofa (Pig)
Sample Types serum, plasma
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig LPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 87-100
1:2 Average % 92
Range % 87-96
1:4 Average % 90
Range % 83-94
1:8 Average % 99
Range % 93-102
Recovery
The recovery of pig LPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 100 91-104
EDTA plasma (n=4) 105 94-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1000 2.246 2.146 2.196 2.024
500 1.591 1.534 1.563 1.391
250 1.127 1.087 1.107 0.935
125 0.885 0.862 0.874 0.702
62.5 0.591 0.578 0.585 0.413
31.2 0.441 0.431 0.436 0.264
15.6 0.301 0.310 0.306 0.134
0 0.174 0.170 0.172  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Secreted
Protein Families AB hydrolase superfamily, Lipase family
Database Links

KEGG: ssc:397537

UniGene: Ssc.95883

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