Pig plasma kallikrein(KLKB1) ELISA Kit

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Code CSB-E17174p
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P03952
Lead Time 3-5 working days
Abbreviation KLKB1
Protein Biological Process 1 Blood Coagulation
Target Name plasma kallikrein(KLKB1)
Alias KLK3, PPK, Fletcher factor|kininogenin|plasma kallikrein B1|plasma kallikrein heavy chain|plasma kallikrein light chain
Species Sus scrofa (Pig)
Protein Biological Process 3 Blood coagulation
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.
HGNC 6364
RGD 2971
MGI 892021
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 98
Range % 94-106
1:200 Average % 89
Range % 83-94
1:400 Average % 103
Range % 98-107
1:800 Average % 86
Range % 82-91
Recovery
The recovery of pig KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 88-98
EDTA plasma (n=4) 103 98-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
1000 0.086 0.088 0.087
500 0.108 0.113 0.111
250 0.154 0.161 0.158
125 0.232 0.243 0.238
62.5 0.373 0.388 0.381
31.2 0.651 0.680 0.666
15.6 0.852 0.911 0.882
0 1.821 1.975 1.898
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Function The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Involvement in disease Prekallikrein deficiency (PKK deficiency)
Subcellular Location Secreted
Protein Families Peptidase S1 family, Plasma kallikrein subfamily
Database Links

HGNC: 6371

OMIM: 229000

KEGG: hsa:3818

STRING: 9606.ENSP00000264690

UniGene: Hs.237642

Pathway Complement and coagulation cascades

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