Pig regulated on activation in normal T-cell expressed and secreted,RANTES ELISA Kit

Instructions
Code CSB-E13179p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) ligand 5
Alternative Names CCL5 ELISA Kit; SCYA5 ELISA Kit; C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit; Fragment ELISA Kit
Abbreviation CCL5
Uniprot No. Q29288
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 100
Range % 95-104
1:2 Average % 101
Range % 91-105
1:4 Average % 92
Range % 84-95
1:8 Average % 106
Range % 98-110
Recovery
The recovery of pig RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 94-106
EDTA plasma (n=4) 90 84-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.233 2.313 2.273 2.163
10 1.769 1.814 1.792 1.682
5 1.347 1.434 1.391 1.281
2.5 0.965 1.004 0.985 0.875
1.25 0.517 0.532 0.525 0.415
0.625 0.377 0.384 0.381 0.271
0.312 0.251 0.261 0.256 0.146
0 0.109 0.111 0.110  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

STRING: 9823.ENSSSCP00000028561

UniGene: Ssc.22030

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