Porcine hypoxia-inducible factor 1α,HIF-1α ELISA Kit

Instructions
Code CSB-E11288p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name hypoxia-inducible factor 1α,HIF-1
Abbreviation HIF-1
Uniprot No. F1SRU5
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 156.25 pg/mL-10000 pg/mL
Sensitivity 39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays):CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 91    
Range % 86-95    
1:2 Average % 102    
Range % 97-107    
1:4 Average % 91    
Range % 85-97    
1:8 Average % 97    
Range % 91-103    
Recovery
The recovery of pig HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 89-98    
EDTA plasma (n=4) 92 88-96    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
10000 2.302 2.401 2.352 2.179    
5000 1.726 1.736 1.731 1.558    
2500 1.043 1.002 1.023 0.850    
1250 0.561 0.552 0.557 0.384    
625 0.405 0.412 0.409 0.236    
312.5 0.323 0.332 0.328 0.155    
156.25 0.248 0.251 0.250 0.077    
0 0.176 0.170 0.173      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.