Rabbit E-Selectin ELISA kit

Code CSB-E07322Rb
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name selectin E
Alternative Names RP1-117P20.2, CD62E, ELAM, ELAM1, ESEL, LECAM2, endothelial adhesion molecule 1|leukocyte endothelial cell adhesion molecule 2
Abbreviation SELE
Uniprot No. P27113
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit E-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 87-102
1:2 Average % 94
Range % 90-103
1:4 Average % 95
Range % 90-100
1:8 Average % 93
Range % 84-98
The recovery of rabbit E-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 91-110
EDTA plasma (n=4) 95 89-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.714 2.726 2.720 2.550
20 2.153 2.112 2.133 1.963
10 1.435 1.467 1.451 1.281
5 0.926 0.935 0.931 0.761
2.5 0.518 0.537 0.528 0.358
1.25 0.386 0.373 0.380 0.210
0.625 0.257 0.252 0.255 0.085
0 0.171 0.168 0.170  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Selectin/LECAM family
Database Links

KEGG: ocu:100009151

UniGene: Ocu.1871

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