Rabbit E-Selectin ELISA kit

Code CSB-E07322Rb
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P27113
Lead Time 3-5 working days
Abbreviation SELE
Protein Biological Process 1 Cell Adhesion
Target Name selectin E
Alias RP1-117P20.2, CD62E, ELAM, ELAM1, ESEL, LECAM2, endothelial adhesion molecule 1|leukocyte endothelial cell adhesion molecule 2
Species Oryctolagus cuniculus (Rabbit)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Target Details This protein is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.
HGNC 10718
RGD 3654
MGI 98278
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit E-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 87-102
1:2 Average % 94
Range % 90-103
1:4 Average % 95
Range % 90-100
1:8 Average % 93
Range % 84-98
The recovery of rabbit E-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 91-110
EDTA plasma (n=4) 95 89-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.714 2.726 2.720 2.550
20 2.153 2.112 2.133 1.963
10 1.435 1.467 1.451 1.281
5 0.926 0.935 0.931 0.761
2.5 0.518 0.537 0.528 0.358
1.25 0.386 0.373 0.380 0.210
0.625 0.257 0.252 0.255 0.085
0 0.171 0.168 0.170  
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Function Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Selectin/LECAM family
Database Links

KEGG: ocu:100009151

UniGene: Ocu.1871

Pathway TNF signaling pathway
AGE-RAGE signaling pathway in diabetic complications

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