Rabbit High mobility group protein B1,HMGB-1 ELISA Kit

Code CSB-E08894Rb
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. B7NZ89
Lead Time 3-5 working days
Abbreviation HMGB-1
Target Name High mobility group protein B1,HMGB-1
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 10 ng/mL-100 ng/mL
Sensitivity 2 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 86-103    
1:2 Average % 95    
Range % 88-105    
1:4 Average % 95    
Range % 91-97    
1:8 Average % 93    
Range % 87-98    
Recovery
The recovery of rabbit HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 92 87-99    
EDTA plasma (n=4) 91 85-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
100 2.143 2.159 2.151      
57 1.142 1.155 1.149      
22.8 0.336 0.341 0.339      
11.4 0.154 0.158 0.156      
5.7 0.086 0.087 0.087      
References
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