Rabbit Interleukin 1β,IL-1β ELISA Kit

Code CSB-E06900Rb
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 1, beta
Alternative Names
IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit; Lymphocyte proliferation-potentiating factor ELISA kit
Abbreviation
IL1B
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %98
Range %85-105
1:2Average %95
Range %85-103
1:4Average %94
Range %82-100
1:8Average %95
Range %85-105
Recovery
The recovery of rabbit IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9682-104
EDTA plasma (n=4)9585-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.852 2.713 2.783 2.674
2502.235 2.245 2.240 2.131
1251.424 1.392 1.408 1.299
62.50.753 0.784 0.769 0.660
31.20.447 0.433 0.440 0.331
15.60.266 0.282 0.274 0.165
7.80.202 0.195 0.199 0.090
00.111 0.106 0.109  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit IL1B ELISA Kit was designed for the quantitative measurement of Rabbit IL1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Citations

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Target Background

Function
(From Uniprot)
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Gene References into Functions
  1. Mild heat shock increased the production of inflammatory cytokines, IL-1beta and IL-6 in rabbit cornea cells. PMID: 23330884
  2. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
  3. IL-1beta induced a significant reduction in the relative intrinsic activity of GLUT5 and in this decrease are involved NO signal pathways; blockage of D-fructose intestinal uptake by IL-1beta may play an essential role in the pathophysiology of septic shock. PMID: 20803526
  4. The inhibitory effect of IL-1beta on D-galactose absorption across mucosal side of enterocyte could be mediated by the activation of several kinases and nuclear factor kappa B. PMID: 22759965
  5. Glucosamine hydrochloride treatment can can partially decrease the expression levels of IL-1 beta and increase the expression levels of TGF-beta 1, which delays the development of osteoarthritis. PMID: 20369526
  6. In this study evidence is provided that exogenous PGF2alpha differentially modulates luteal expression of IL1B transcripts depending on luteal stage. PMID: 20427144
  7. results revealed that a transient episode of raised-intensity phonation causes a significant increase in vocal fold inflammatory mRNA expression - IL-1beta,COX-2, and TGFbeta1 PMID: 20879195

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Subcellular Location
Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
Protein Families
IL-1 family
Database Links
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