Target Name
Macrophage Inflammatory Protein-1α,MIP-1α
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
105 |
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| Range % |
93-109 |
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| 1:2 |
Average % |
110 |
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| Range % |
97-114 |
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| 1:4 |
Average % |
88 |
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| Range % |
83-93 |
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| 1:8 |
Average % |
95 |
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| Range % |
85-98 |
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Recovery
| The recovery of rabbit MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
105 |
95-109 |
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| EDTA plasma (n=4) |
101 |
91-105 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.803 |
2.736 |
2.770 |
2.629 |
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| 250 |
2.362 |
2.455 |
2.409 |
2.268 |
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| 125 |
1.743 |
1.862 |
1.803 |
1.662 |
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| 62.5 |
1.123 |
1.151 |
1.137 |
0.996 |
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| 31.25 |
0.730 |
0.744 |
0.737 |
0.596 |
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| 15.6 |
0.550 |
0.537 |
0.544 |
0.403 |
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| 7.8 |
0.306 |
0.312 |
0.309 |
0.168 |
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| 0 |
0.139 |
0.143 |
0.141 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
