Rabbit Polymeric immunoglobulin receptor(PIGR) ELISA kit

Instructions
Code CSB-EL017981RB
Size 96T,5×96T,10×96T
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Product Details

Target Name polymeric immunoglobulin receptor
Alternative Names PIGR ELISA Kit; Polymeric immunoglobulin receptor ELISA Kit; PIgR ELISA Kit; Poly-Ig receptor) [Cleaved into: Secretory component] ELISA Kit
Abbreviation PIGR
Uniprot No. P01832
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 31.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PIGR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-95
1:2 Average % 96
Range % 89-110
1:4 Average % 93
Range % 90-96
1:8 Average % 95
Range % 89-105
Recovery
The recovery of rabbit PIGR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-105
EDTA plasma (n=4) 97 90-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
2000 2.734 2.759 2.747 2.620
1000 2.484 2.463 2.474 2.347
500 1.902 1.893 1.898 1.771
250 1.270 1.252 1.261 1.134
125 0.704 0.715 0.710 0.583
62.5 0.463 0.455 0.459 0.332
31.25 0.291 0.296 0.294 0.167
0 0.125 0.128 0.127  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
Subcellular Location Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Secretory component: Secreted
Database Links

KEGG: ocu:100328593

STRING: 9986.ENSOCUP00000012314

UniGene: Ocu.3262

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