Rabbit Pulmonary surfactant-associated protein A(SFTPA1) ELISA kit

Code CSB-EL021168RB
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
surfactant protein A1
Alternative Names
SFTPA1 ELISA Kit; SFTP1 ELISA Kit; SFTPA ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
Abbreviation
SFTPA1
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SFTPA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %82-96
1:2Average %107
Range %90-111
1:4Average %90
Range %85-94
1:8Average %91
Range %85-95
Recovery
The recovery of rabbit SFTPA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9082-95
EDTA plasma (n=4)9990-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.110 1.991 2.051 1.950
501.813 1.688 1.751 1.650
251.382 1.484 1.433 1.332
12.51.053 1.028 1.041 0.940
6.250.726 0.705 0.716 0.615
3.120.536 0.528 0.532 0.431
1.560.309 0.302 0.306 0.205
00.098 0.104 0.101
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit SFTPA1 ELISA Kit was designed for the quantitative measurement of Rabbit SFTPA1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

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Target Background

Function
(From Uniprot)
In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
Protein Families
SFTPA family
Database Links
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