Rabbit beta-Endorphin(β-EP) ELISA Kit

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Code CSB-E13088Rb
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation β-EP
Target Name beta-Endorphin(β-EP)
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 87-102
1:2 Average % 96
Range % 90-103
1:4 Average % 89
Range % 86-96
1:8 Average % 95
Range % 89-98
Recovery
The recovery of rabbit β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 89-105
EDTA plasma (n=4) 95 90-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
400 2.215 2.225 2.220 2.010
200 1.615 1.587 1.601 1.391
100 1.168 1.217 1.193 0.983
50 0.782 0.763 0.773 0.563
25 0.625 0.603 0.614 0.404
12.5 0.446 0.435 0.441 0.231
6.25 0.317 0.308 0.313 0.103
0 0.213 0.207 0.210  
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