Rat α-melanocyte stimulating hormone (α-MSH) ELISA kit

Instructions
Code CSB-EQ027809RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name α-melanocyte stimulating hormone (α-MSH)
Abbreviation α-MSH
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 5 pg/mL-1000 pg/mL
Sensitivity 2.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Biochemicals
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-99
1:2Average %89
Range %81-100
1:4Average %97
Range %91-105
1:8Average %95
Range %87-102
Recovery
The recovery of rat α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9391-100
EDTA plasma (n=4)9588-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
10000.181 0.176 0.179
2500.331 0.325 0.328
800.630 0.620 0.625
201.064 1.052 1.058
51.418 1.427 1.423
02.586 2.577 2.582
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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