Product Details

Description

This Rat ENO1 ELISA Kit was designed for the quantitative measurement of Rat ENO1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

Target Name

α-enolase

Alternative Names

Eno1 ELISA Kit; Eno-1Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit

Abbreviation

ENO1

Uniprot No.

P04764

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat α-enolase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	102
	Range %	97-107
1:4	Average %	101
	Range %	95-105
1:8	Average %	94
	Range %	91-98

Recovery

The recovery of rat α-enolase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-108
EDTA plasma (n=4)	87	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.191	2.275	2.233	2.111
20	1.448	1.538	1.493	1.371
10	0.921	0.963	0.942	0.820
5	0.563	0.572	0.568	0.446
2.5	0.334	0.341	0.338	0.216
1.25	0.231	0.245	0.238	0.116
0.625	0.173	0.179	0.176	0.054
0	0.121	0.122	0.122

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Gene References into Functions	gamma-enolase may signal and correlate lung tissue damage well before obvious physical pathological tissue damage and might be a candidate diagnostic and/or prognostic marker. PMID: 29728894 The present study aimed to investigate the change of alpha-enolase during cardiac hypertrophy and explore its role in this pathological process. PMID: 28910549 alpha-enolase has a catalytically independent role in inducing cardiomyocyte apoptosis and mitochondrial dysfunction. PMID: 25446184 this research suggests that the protective effect of Hsc70 on the cardiomyocytes against oxidative stress is partly associated with its interaction with alpha-enolase. PMID: 21958194 alpha-Enolase binds to RNA PMID: 21621582 The alpha-enolase hyper-phosphorylation in hypertrophied left ventricle was accompanied by decreased enzymatic activity. PMID: 18468517 Data show that the expression of alpha-enolase in the left ventricle of the rats during normal and pathological cardiac development is different. PMID: 19450578 enolase alpha was up-regulated in left ventricle of spontaneously hypertensive rats. PMID: 16485256 Show More Hide All
Subcellular Location	Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Protein Families	Enolase family
Tissue Specificity	Expressed in flagella of epididymal sperm. The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma
Database Links	KEGG: rno:24333 STRING: 10116.ENSRNOP00000024106 UniGene: Rn.117044

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Code	CSB-E17439r
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Rat α-enolase ELISA Kit

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