Rat β2-microglobulin,BMG/β2-MG ELISA Kit

Instructions
Code CSB-E11298r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name beta-2-microglobulin
Alternative Names B2MBeta-2-microglobulin ELISA kit
Abbreviation B2M
Uniprot No. P01885
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.039 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %82-92
1:2Average %96
Range %92-101
1:4Average %100
Range %95-105
1:8Average %90
Range %85-95
Recovery
The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-104
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2AverageCorrected
102.645 2.678 2.662 2.515
51.855 1.896 1.876 1.729
2.51.256 1.263 1.260 1.113
1.250.834 0.869 0.852 0.705
0.6250.487 0.489 0.488 0.341
0.3120.325 0.353 0.339 0.192
0.1560.261 0.284 0.273 0.126
00.145 0.149 0.147  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Subcellular Location Secreted.
Protein Families Beta-2-microglobulin family
Database Links

STRING: 9986.ENSOCUP00000014707

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