Rat 3-hydroxy-3-methylglutaryl-Coenzyme A synthase, HMGCS ELISA Kit

Code CSB-E13650r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P17425
Lead Time 3-5 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation HMGCS1
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
Alias HMGCS, MGC90332, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase|hydroxymethylglutaryl-CoA synthase 1
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Cholesterol biosynthesis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat HMGCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 95-103
1:2 Average % 87
Range % 83-90
1:4 Average % 102
Range % 98-106
1:8 Average % 85
Range % 81-89
Recovery
The recovery of rat HMGCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 89-96
EDTA plasma (n=4) 107 103-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.776 2.801 2.789 2.661
10 2.307 2.242 2.275 2.147
5 1.708 1.698 1.703 1.575
2.5 1.198 1.244 1.221 1.093
1.25 0.738 0.745 0.742 0.614
0.625 0.419 0.432 0.426 0.298
0.312 0.234 0.227 0.231 0.103
0 0.127 0.129 0.128  
References
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Function This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Subcellular Location Cytoplasm
Protein Families HMG-CoA synthase family
Database Links

KEGG: rno:29637

STRING: 10116.ENSRNOP00000023554

UniGene: Rn.5106

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