Rat Adrenocorticotropic hormone receptor(MC2R) ELISA kit

Code CSB-EL013559RA
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q8CIX6
Abbreviation MC2R
Alias ACTHR, MGC125798, ACTH receptor|MC2 receptor|OTTHUMP00000162512|corticotropin receptor|melanocortin 2 receptor
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Availability 7-14 working days
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target Details MC2R encodes one member of the five-member G-protein associated melanocortin receptor family. Melanocortins (melanocyte-stimulating hormones and adrenocorticotropic hormone) are peptides derived from pro-opiomelanocortin (POMC). MC2R is selectively activated by adrenocorticotropic hormone, whereas the other four melanocortin receptors recognize a variety of melanocortin ligands. Mutations in MC2R can result in familial glucocorticoid deficiency.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MC2R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 86-96
1:2 Average % 102
Range % 98-105
1:4 Average % 91
Range % 85-97
1:8 Average % 95
Range % 91-100
Recovery
The recovery of rat MC2R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.422 2.563 2.493 2.365
500 1.966 1.832 1.899 1.771
250 1.279 1.331 1.305 1.177
125 0.769 0.778 0.774 0.646
62.5 0.456 0.464 0.460 0.332
31.25 0.281 0.273 0.277 0.149
15.6 0.204 0.208 0.206 0.078
0 0.127 0.128 0.128  

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