Rat Apolipoprotein A-II(APOA2) ELISA kit

Instructions
Code CSB-EL001915RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein A-II
Alternative Names Apoa2Apolipoprotein A-II ELISA kit; Apo-AII ELISA kit; ApoA-II ELISA kit; Apolipoprotein A2) [Cleaved into: Proapolipoprotein A-II ELISA kit; ProapoA-II)] ELISA kit
Abbreviation APOA2
Uniprot No. P04638
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 ng/mL-1200 ng/mL
Sensitivity 4.7 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat APOA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 102
Range % 97-106
1:2000 Average % 94
Range % 88-98
1:4000 Average % 97
Range % 90-101
1:8000 Average % 100
Range % 94-105
Recovery
The recovery of rat APOA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 95-104
EDTA plasma (n=4) 95 87-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
1200 0.183 0.181 0.182
600 0.315 0.308 0.312
300 0.574 0.565 0.570
150 0.933 0.887 0.910
75 1.389 1.368 1.379
37.5 1.894 1.853 1.874
18.75 2.204 2.269 2.237
0 2.556 2.473 2.515
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
Gene References into Functions
  1. ApoAII is efficiently reabsorbed in kidney proximal tubules in relation to its plasma concentration PMID: 17652309
Subcellular Location Secreted
Protein Families Apolipoprotein A2 family
Tissue Specificity Plasma.
Database Links

KEGG: rno:25649

STRING: 10116.ENSRNOP00000004662

UniGene: Rn.89304

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