Rat Aquaporin 5,AQP-5 ELISA Kit

Instructions
Code CSB-E08258r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name aquaporin 5
Alternative Names Aqp5Aquaporin-5 ELISA kit; AQP-5 ELISA kit
Abbreviation AQP5
Uniprot No. P47864
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.18 ng/mL-12 ng/mL
Sensitivity 0.05 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AQP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 105    
Range % 101-109    
1:2 Average % 96    
Range % 94-98    
1:4 Average % 89    
Range % 83-95    
1:8 Average % 93    
Range % 89-97    
Recovery
The recovery of rat AQP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 101 99-104    
EDTA plasma (n=4) 90 85-96    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
12 2.568 2.678 2.623 2.486    
6 1.921 1.989 1.955 1.818    
3 1.184 1.174 1.179 1.042    
1.5 0.647 0.655 0.651 0.514    
0.75 0.449 0.461 0.455 0.318    
0.37 0.285 0.287 0.286 0.149    
0.18 0.213 0.221 0.217 0.080    
0 0.134 0.140 0.137      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells.
Gene References into Functions
  1. These findings support the involvement of AQP5 in oxidative stress and suggest AQP5 modulation by phosphorylation as a novel tool for therapeutics. PMID: 27983600
  2. hypothyroid state induced significant increases in the expression of lung phospho-MLC and AQP5 proteins. In contrast, the hyperthyroid state caused moderate decreases in lung phospho-MLC and AQP5 proteins PMID: 25649359
  3. AQP5 translocates to the nuclear membrane and may trigger the movement of water, inducing shrinkage of the nucleus and the start of nuclear functions. PMID: 25603543
  4. These results lead us to propose that oxytocin selectively influences the expression of Aqp5 at the end of pregnancy, and may participate in events that lead to parturition in the rat. PMID: 24644013
  5. After ovulation AQP5 is involved in the transcellular movement of water in the oviduct ampulla and oocytes in rats. PMID: 24192214
  6. the present study has shown that AQP5 participates in the development of lung edema induced by bacterial endotoxin. PMID: 23538169
  7. The results of this study indicated that AQP5 might be an important water channel in astrocytes that is differentially expressed during various brain injuries. PMID: 23922257
  8. AQP5 is a significant component of lens fiber cell membranes, representing the second most abundant water channel in these cells. PMID: 23313152
  9. AQP5 is the main pathway for water transport in the acinar cells. PMID: 22903161
  10. AQP5 expression in the apical membrane of acinar cells was less prominent in the experimental rats. Findings suggest that unilateral molar extraction influences the function of water transport in the rat submandibular salivary gland. PMID: 22424887
  11. Aquaporin-5 was present at the apical face of the olfactory epithelium, completing a water transport pathway to the surface of the epithelium. PMID: 21745799
  12. AQP5 immunolabeling of the RPE was increased in retinal slices from diabetic rats in comparison to slices from control rats. PMID: 21851171
  13. AQP5 production is regulated through the cAMP-PKA/CREB pathway in rat nasal epithelial cells. PMID: 21991573
  14. Glucocorticoid can down-regulate the expression of AQP5, but H1-receptor antagonist can not reduce the expression of AQP5 in in nasal mucosa of rats with experimental allergic rhinitis. PMID: 20128285
  15. Data suggest that Lack of AQP5 translocation in the salivary gland in response to a muscarinic agonist and downregulation of AQP5 protein might lead to diabetic xerostomia. PMID: 21295117
  16. Replacement of Gly103 with Asp103 in rat AQP5 did not affect water permeability. PMID: 21138418
  17. The hypersecretion of nasal mucosa glands has a close relationship with the high expression of AQP5 in allergic rhinitis. PMID: 15952563
  18. The significant decrease in AQP5 may be an important factor of pulmonary edema formation in hyperoxia-induced lung injury. PMID: 16887054
  19. We conclude that the presence of AQP5 in plasma membranes of sweat glands is essential for secretion, providing potential insight into mechanisms underlying mammalian thermoregulation, tactile sensitivity, and the pathophysiology of hyperhidrosis PMID: 11773623
  20. Genetic variation in the expression of AQP5 in rats. PMID: 12466944
  21. AQP-5 is localized predominantly in apical plasma membrane, and neither onset nor cessation of secretion is associated in vivo with any significant short-term translocation of AQP-5 between intracellular structures and the apical plasma membrane. PMID: 14988067
  22. This review focuses on mechanisms of AQP5 translocation in the interlobular duct cells of parotid glands of normal, diabetic, and senescent rats following activation of M3 muscarinic acetylcholine receptors. PMID: 15548853
  23. AQP5 localizes in lipid rafts and activation of the M3 mAChRs or alpha1-adrenoceptors induced its translocation together with the lipid rafts to the APM in the interlobular ducts of rat parotid glands. PMID: 16712780
  24. This mutation may have resulted in an abnormal membrane insertion or ineffective trafficking of AQP5. PMID: 16901987
  25. In conditions that increased AQP5 levels, cells showed a faster cell swelling and a more complete volume recovery following hyposmotic exposure PMID: 17021794
  26. Hypertonic induction of both AQP5 and VEGF is transcriptionally regulated and mediated, at least in part, by HIF-1alpha, suggesting a novel role for HIF-1alpha in modulating cellular adaptive responses to osmotic stress. PMID: 17108010
  27. AQP5 protein is necessary for sweat secretion, and that the expression of AQP5 in rat sweat glands is independent of sympathetic innervation PMID: 17380350
  28. 4.3-kb Aqp5 promoter/enhancer directs strong cell-specific transgene expression in salivary gland and low-level AT1 cell-specific expression in the lung. PMID: 18448628
  29. These results suggest the possible regulation of the AQP5 protein level in the submandibular gland by the parasympathetic nerves/M3 muscarinic receptor agonist and imply the involvement of lysosomal enzymes in this regulation. PMID: 18450949
  30. GATA-6 mediates transcriptional activation of aquaporin-5 through interactions with Sp1. PMID: 18768929
  31. the suppression of PACE4 expression by siRNAs resulted in decreased expression of AQP5 and inhibition of the branching morphogenesis in the present organ culture system. PMID: 19013448
  32. gas channels exhibit selectivity for CO(2) vs. NH(3) permeability, demonstrating the sequence AQP4 congruent with AQP5 > AQP1 > AmtB > RhAG. PMID: 19273840
  33. A disturbance of AQP5 translocation may be involved in the pathogenesis of X-ray irradiation-induced xerostomia. PMID: 19673936

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Subcellular Location Apical cell membrane, Multi-pass membrane protein
Protein Families MIP/aquaporin (TC 1.A.8) family
Tissue Specificity Salivary glands, lacrimal glands, corneal epithelium in eye, trachea and lung.
Database Links

KEGG: rno:25241

STRING: 10116.ENSRNOP00000024102

UniGene: Rn.10066

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