Name: Rat Beta-type platelet-derived growth factor receptor(PDGFRB) ELISA kit
Brand: CUSABIO
SKU: CSB-EL017713RA

Product Details

Target Name

platelet-derived growth factor receptor, beta polypeptide

Alternative Names

Pdgfrb ELISA Kit; Pdgfr ELISA Kit; Pdgfr1 ELISA Kit; Platelet-derived growth factor receptor beta ELISA Kit; PDGF-R-beta ELISA Kit; PDGFR-beta ELISA Kit; EC 2.7.10.1 ELISA Kit; Beta platelet-derived growth factor receptor ELISA Kit; Beta-type platelet-derived growth factor receptor ELISA Kit; CD140 antigen-like family member B ELISA Kit; Platelet-derived growth factor receptor 1 ELISA Kit; PDGFR-1 ELISA Kit; CD antigen CD140b ELISA Kit

Abbreviation

PDGFRB

Uniprot No.

Q05030

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGFRB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	96
	Range %	91-100
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	89-97

Recovery

The recovery of rat PDGFRB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	101	95-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.464	2.548	2.506	2.318
1500	1.618	1.716	1.667	1.479
750	1.173	1.103	1.138	0.950
375	0.657	0.695	0.676	0.488
187.5	0.492	0.479	0.486	0.298
94	0.331	0.352	0.342	0.154
47	0.249	0.237	0.243	0.055
0	0.185	0.191	0.188

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

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Target Data

Function	Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor (By similarity).
Gene References into Functions	Developmental 2,3,7,8-tetrachlorodibenzo-p-dioxin and high fat diet exposure induced changes in body growth and feeding behaviors that may be mediated by brain Pdgfrb level changes: Pdgfrb levels in the hippocampus and striatum were inversely correlated with increases in body length, while changes in Pdgfrb in the amygdala and nucleus accumbens were significantly correlated to body weight gain or body mass index. PMID: 28891521 Our data provide the first evidence for a critical sequential role for mTOR-Notch3-PDGF-beta receptor signaling in the pathogenesis of chronic neonatal pulmonary hypertension. PMID: 28759157 Data indicate transforming growth factor beta (TGFbeta) involvement in early activation of platelet-derived growth factor receptor beta (PDGFRbeta)+ pericytes in aristolochic acid induced acute kidney injury. PMID: 27379382 These data suggest that miR182 may be a novel smooth muscle cell phenotypic marker and a modulator that may be used to prevent smooth muscle cell dedifferentiation via FGF9/PDGFRbeta signaling. PMID: 28259995 Data suggest that expression of microRNA-9 (Mir9) in cardiac fibroblasts is involved in regulation of expression of Pdgfr-beta (platelet-derived growth factor beta receptor) and in prevention of cardiac fibrosis; here, overexpression of Mir9 inhibits cardiac fibrosis in primary cardiac fibroblasts by targeting Pdgfr-beta. PMID: 26896308 dihydroartemisinin protects the liver from injury and fibrogenesis via disruption of the PDGF-betaR/ERK pathway PMID: 27038258 IL-1beta-induced MMP-9 expression is mediated through a c-Src-mediated transactivation of PDGFR/PI3K/Akt cascade linking to p47(phox)/NADPH oxidase 2 (Nox2)/reactive oxygen species signaling pathway. PMID: 25159478 The mRNA and protein expression levels of PDGFRbeta were upregulated in vascular smooth muscle cells after a 24- and 48-h incubation with Deoxycholic acid, respectively. PMID: 25866311 miR-30c regulates PDGFRB expression by its direct binding the 3'UTR of PDGFRB mRNA. PMID: 25882492 Pro-inflammatory cytokines both initiate and maintain a growth response in colonic smooth muscle cells via PDGF-Rbeta. PMID: 24417820 These results suggest that gonocyte and F9 cell differentiation is regulated via cross talk between retinoic acid and PDGFRs using different downstream pathways. PMID: 25380237 PPARgamma inhibited angiogenic signal transduction through transrepression of PDGF-beta receptor leading to reduced hepatic stellate cell motility. PMID: 24259663 During myocardial fibrosis of desoxycorticosterone induced salt-sensitive hypertensive rats, PDGFRalpha acts at early stage, but PDGFRbeta functions in the whole process PMID: 24427322 SHP-1 as an off-switch of PDGFR signaling appears to emerge as a valuable molecular target to trigger as to prevent hepatic stellate cells (HSC) proliferation and the fibrogenic effects of HSC activation. PMID: 24140598 bFGF promote the proliferation and migration of endothelial progenitor cells, the effects of bFGF being implemented by activating ERK signalling through the expression of Pdgfrb. PMID: 22731705 Clitocybin B suppressed the PDGF-Rbeta activation in PDGF-BB signaling cascade. PMID: 22166585 we show that platelet-derived growth factor receptor-beta (PDGFR-beta)-mediated signaling plays a key role in morphine tolerance. PMID: 22344297 PDGFRbeta-Akt signaling in brain pericytes may play various important roles leading to neuroprotection after ischemic stroke. PMID: 22272762 PDGF-beta receptor and PKC have no effect on angiotensin II-induced JAK2 and STAT1 phosphorylation in vascular smooth muscle cells under high glucose condition PMID: 21929289 VEGFR2+PDGFRbeta+ circulating precursor cells participate in capillary restoration after hyperoxia acute lung injury PMID: 19426150 In the hypertensive lung, PDGF-Rbeta was highly expressed by fibroblasts developing as perivascular cells. PMID: 16971352 This study demonstrates that PDGFbeta receptors act via an interaction with Abl kinase and Rho kinase to regulated cytoskeletal regulation of NMDA receptor channels in CA1 pyramidal neurons. PMID: 19077273 Data show that PTPepsilonM negatively regulates PDGFRbeta-mediated signaling pathways that are crucial for the pathogenesis of atherosclerosis, and PTPepsilonM may be involved in diabetes-accelerated atherosclerosis. PMID: 20686073 PDGFbeta receptor is transcriptionally regulated by NF-Y and p73 PMID: 12167641 Results show that peroxide stimulates phosphorylation of the platelet-derived growth factor receptor beta, and that this phosphorylation requires protein kinase C delta and c-src. PMID: 12226102 expression of novel V1-PDGFRbeta is tightly regulated during differentiation and that it may play an active role in germ cell differentiation PMID: 17303670 PDGF can further increase PDGFR auto-phosphorylation despite strong Src kinase activity, but diffuse downstream activation of PI3-K by Src abrogates cell polarization and chemotaxis: "signalling requires silence". PMID: 17335807 LOX activity is essential to generate optimal chemotactic sensitivity of cells to chemoattractants by oxidizing specific cell surface proteins, such as PDGFR-beta. PMID: 18586678 Gene transfer of redox factor-1 inhibits neointimal formation: involvement of platelet-derived growth factor-beta receptor signaling via the inhibition of the reactive oxygen species-mediated Syk pathway. PMID: 19038866 Differential effects of red and white wines on inhibition of the platelet-derived growth factor receptor: impact of the mash fermentation. PMID: 19074160 Data indicate that indoxyl sulfate induces the migration as well as proliferation of VSMCs through PDGF-beta receptors and that ROS generation is critically involved in this process, which promotes the development of atherosclerosis. PMID: 19494236 Show More Hide All
Subcellular Location	Cell membrane, Single-pass type I membrane protein, Cytoplasmic vesicle, Lysosome lumen
Protein Families	Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Database Links	KEGG: rno:24629 STRING: 10116.ENSRNOP00000060534 UniGene: Rn.98311

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Check Code：

Code	CSB-EL017713RA
Size	96T,5×96T,10×96T
Trial Size
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