Rat C-telopeptide of type Ⅰ collagen,CTX-Ⅰ ELISA Kit

Code CSB-E12776r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
C-telopeptide of type Ⅰ collagen,CTX-
Alternative Names
C-telopeptide of type 1 collagen,CTX-1,C-telopeptide of type I collagen,CTX-I
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
25 pg/mL-2000 pg/mL
31.25 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 89-97  
1:2 Average % 92  
Range % 88-96  
1:4 Average % 98  
Range % 94-102  
1:8 Average % 84  
Range % 80-88  
The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-102  
EDTA plasma (n=4) 86 82-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.353 0.354 0.354    
750 0.468 0.475 0.472    
375 0.616 0.605 0.611    
125 1.011 0.995 1.003    
25 1.512 1.594 1.553    
0 2.013 2.114 2.064    
Materials provided
  • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-human CTX-I antibody.
  • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • Anti-rat CTX-I Antibody 1 x 6 ml -- Act as the detection antibody.
  • HRP-conjugated CTX-I 1 x 6 ml -- Bind to the CTX-I antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
  • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
  • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
  • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
  • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Rat CTX- ELISA Kit was designed for the quantitative measurement of Rat CTX- protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 25 pg/mL-2000 pg/mL and the sensitivity is 31.25 pg/mL.


Customer Reviews and Q&A

 Customer Reviews
Average Rating:
4.5 - 2 reviews

Submit a Review here

Sample type: Serum

Sample species: Rat

Review: Serum biomarkers of bone formation, N-terminal propeptide of type I procollagen (PINP) and of bone resorption, C-terminal telopeptide of type I collagen (CTX-I) indicated that structural bone changes observed following injury are mainly caused by the decreased bone resorption with a mild change in bone formation. Values represent the mean ± standard deviation (SD). Each group consisted of 6 rats.*P< 0.05 compared to Sham by unpaired t-test; ^ P < 0.05 compared to Control; #P < 0.05 compared to CS; $< 0.05 compared to CS+IB.

By Anonymous

Sample type: Serum

Sample species: Rat

Review: 1.Ctrl; 2.Sham(no ovariectomy); 3.Ovariectomy; 4.Ovariectomy+E2; 5.Ovariectomy+sample1; 6.Ovariectomy+sample2; 7.Ovariectomy+sample3; 8.Ovariectomy+sample4

By Anonymous


I am writing to ask you a question about how to experiment.
I'd like to experiment with kit #CSB-E12776r.
Sample type is serum.
When diluting a sample, what solution would you recommend?

Thanks for your inquiry. Regarding this item,#CSB-E12776r,there is no indeed recommadated dilution factors.Usually there is no need for dilutions and you can do pre-testing and research for the best dilution factor if you need dilutions.Series 0;1:10;1:100 is advised.(you can consider use PBS as diluent solution).
If any further questions,pls do not hesitate to tell me.

What's the antibody information of this kit?

Thanks for your inquiry. Pls check our information: CSB-E12776r: Coated anti. Goat against rabbit secondary antibody; Antigen Specific anti: Rabbit polyclonal antibody; Pls let me know if you have any further questions. Thank you.
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