Rat Caspase-8,Casp-8 ELISA Kit

Instructions
Code CSB-E14912r
Size 96T,5×96T,10×96T

Product Details

Target Name Caspase-8,Casp-8
Alternative Names Caspase-8 (CASP-8) (EC 3.4.22.61) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10], Casp8
Abbreviation Casp-8
Uniprot No. Q9JHX4
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 103-108
1:2 Average % 98
Range % 93-102
1:4 Average % 93
Range % 84-98
1:8 Average % 95
Range % 89-98
Recovery
The recovery of rat Casp-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 99-107
EDTA plasma (n=4) 92 83-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 1.996 2.033 2.015 1.821
10 1.444 1.524 1.484 1.290
5 0.923 0.955 0.939 0.745
2.5 0.658 0.625 0.642 0.448
1.25 0.468 0.474 0.471 0.277
0.625 0.365 0.385 0.375 0.181
0.312 0.307 0.302 0.305 0.111
0 0.189 0.199 0.194  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Database Links

KEGG: rno:64044

STRING: 10116.ENSRNOP00000016613

UniGene: Rn.54474

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