Rat Caspase-8,Casp-8 ELISA Kit

Instructions
Code CSB-E14912r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Caspase-8,Casp-8
Alternative Names Casp8Caspase-8 ELISA kit; CASP-8 ELISA kit; EC 3.4.22.61) [Cleaved into: Caspase-8 subunit p18; Caspase-8 subunit p10] ELISA kit
Abbreviation Casp-8
Uniprot No. Q9JHX4
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 103-108
1:2 Average % 98
Range % 93-102
1:4 Average % 93
Range % 84-98
1:8 Average % 95
Range % 89-98
Recovery
The recovery of rat Casp-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 99-107
EDTA plasma (n=4) 92 83-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 1.996 2.033 2.015 1.821
10 1.444 1.524 1.484 1.290
5 0.923 0.955 0.939 0.745
2.5 0.658 0.625 0.642 0.448
1.25 0.468 0.474 0.471 0.277
0.625 0.365 0.385 0.375 0.181
0.312 0.307 0.302 0.305 0.111
0 0.189 0.199 0.194  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
Gene References into Functions
  1. Hepatocyte apoptosis could promote NAFLD progression; Fas, FasL, and Caspase-8 mRNA activation were important contributing factors to NAFLD. The upregulation of Bax and Bcl-2 expression might be one important mechanism of the apoptosis in NAFLD. PMID: 24938610
  2. Caspase-8 promotes NLRP1/NLRP3 inflammasome activation and IL-1beta production in acute glaucoma. PMID: 25024200
  3. These results suggest that TRAIL inhibits VEGF-induced angiogenesis by increasing caspase-8 activity and subsequently decreasing non-apoptotic signaling functions of procaspase-8, without inducing caspase-3 activation and endothelial cell cytotoxicity. PMID: 24097299
  4. Dietary Chlorella was found to decrease caspase-8 mediated DNA fragmentation induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in colon tissues. PMID: 23466459
  5. activation of caspase-8 in inflamed microglia prevents their death by necroptosis involving RIPK1 PMID: 23386613
  6. Sublytic C5b-9 complexes induce apoptosis of glomerular mesangial cells in rats with Thy-1 nephritis through role of interferon regulatory factor-1-dependent caspase 8 activation. PMID: 22427665
  7. data suggest that caspase-8 does not become activated through an extrinsic but an "intrinsic/intracellular" apoptotic pathway unleashed by the down-regulation of c-FLIP by c-Myc PMID: 21903094
  8. TNF-alpha-induced caspase-8 activation leads to leaky RyR2 channels that contribute to myocardial remodeling after ischemia/reperfusion. PMID: 21788490
  9. The role of N-acetylcysteine (NAC) in coun oxidative stress & apoptosis inhibition induced by stim with hCG in rat Leydig cells was studied. Caspase-8 expression levels were investigated. PMID: 20824644
  10. The expression of caspase-8 in injured brain could be detected in 30 min after injury, increased significantly in 3h, reached its apex in 1d after injury, and remained 4d before decreasing. PMID: 16524172
  11. mRNA level, protein level and activity of caspase-8 were increased in a dose-dependent manner in livers and lungs of rats after SO(2) inhalation. PMID: 19621461
  12. propose that the caspase 8-mediated degradation of plectin represents a critical biochemical event during CCK-induced secretory blockade and pancreatic acinar cell injury PMID: 11841995
  13. Caspase-8 contributes to apoptosis of axotomized rat retinal ganglion cells in vivo. PMID: 12828936
  14. findings suggest FAN, in concert with caspase-8, contributes to an intracellular signaling cascade resulting in lysosomal permeabilization PMID: 15075251
  15. Abetas may induce apoptosis via the activation of caspase-8; oxidative stress is possibly involved in the Abeta-induced cytotoxic effect; these two mechanisms do not act sequentially but, probably, are independent of each other PMID: 16465389
  16. TNF-alpha-induced increase in apoptosis in adult ventricular myocytes results from caspase-8-dependent impairment of mitochondrial integrity PMID: 16730193
  17. CD95L initiates a rapid caspase 8-dependent endosomal acidification PMID: 18045865

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Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Database Links

KEGG: rno:64044

STRING: 10116.ENSRNOP00000016613

UniGene: Rn.54474

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