Rat Cerebral dopamine neurotrophic factor(ARMETL1) ELISA kit

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Code CSB-EL002119RA
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P0C5I0
Lead Time 7-14 working days
Abbreviation ARMETL1
Target Name arginine-rich, mutated in early stage tumors-like 1
Alias ARMETL1, arginine-rich, mutated in early stage tumors-like 1|conserved dopamine neurotrophic factor
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 24913
RGD 1307600
MGI 3606576
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ARMETL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %85-106
1:2Average %89
Range %86-97
1:4Average %90
Range %84-107
1:8Average %93
Range %88-102
Recovery
The recovery of rat ARMETL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9785-105
EDTA plasma (n=4)9683-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.043 2.016 2.030 1.842
51.552 1.514 1.533 1.345
2.51.102 1.127 1.115 0.927
1.250.786 0.823 0.805 0.617
0.6250.528 0.501 0.515 0.327
0.3120.359 0.337 0.348 0.160
0.1560.254 0.243 0.249 0.061
00.186 0.189 0.188
References
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Function Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra.
Subcellular Location Secreted
Protein Families ARMET family
Database Links

KEGG: rno:361276

STRING: 10116.ENSRNOP00000030751

UniGene: Rn.144150

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