Rat Cerebral dopamine neurotrophic factor(ARMETL1) ELISA kit

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Rat Cerebral dopamine neurotrophic factor(ARMETL1) ELISA kit

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Specification
Target

Code

CSB-EL002119RA

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P0C5I0

Lead Time

7-14 working days

Abbreviation

ARMETL1

Target Name

arginine-rich, mutated in early stage tumors-like 1

Alias

ARMETL1, arginine-rich, mutated in early stage tumors-like 1|conserved dopamine neurotrophic factor

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

HGNC

24913

RGD

1307600

MGI

3606576

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ARMETL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	85-106
1:2	Average %	89
	Range %	86-97
1:4	Average %	90
	Range %	84-107
1:8	Average %	93
	Range %	88-102

Recovery

The recovery of rat ARMETL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-105
EDTA plasma (n=4)	96	83-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.043	2.016	2.030	1.842
5	1.552	1.514	1.533	1.345
2.5	1.102	1.127	1.115	0.927
1.25	0.786	0.823	0.805	0.617
0.625	0.528	0.501	0.515	0.327
0.312	0.359	0.337	0.348	0.160
0.156	0.254	0.243	0.249	0.061
0	0.186	0.189	0.188

References

Short-term effect of low-, moderate-, and high-intensity exercise training on cerebral dopamine neurotrophic factor (CDNF) and oxidative stress biomarkers in brain male Wistar rats Hossein Shirvani, et al,Comparative Clinical Pathology,January 5

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Function	Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra.
Subcellular Location	Secreted
Protein Families	ARMET family
Database Links	KEGG: rno:361276 STRING: 10116.ENSRNOP00000030751 UniGene: Rn.144150

Cdnf Protein

Cdnf Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP002119RA CSB-EP002119RA CSB-BP002119RA CSB-MP002119RA	Recombinant Rat Cerebral dopamine neurotrophic factor(Cdnf)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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