Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Code CSB-E08084r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat Col Ⅰ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅰ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL .

Target Name Collagen Type Ⅰ,Col Ⅰ
Alternative Names Collagen Type 1,Col 1,Collagen Type I,Col I
Abbreviation Col Ⅰ
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-88  
1:2 Average % 89  
Range % 84-93  
1:4 Average % 92  
Range % 87-96  
1:8 Average % 95  
Range % 90-99  
Recovery
The recovery of rat Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 88-98  
EDTA plasma (n=4) 98 93-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
5000 2.701 2.762 2.732 2.537  
2500 1.903 1.954 1.929 1.734  
1250 1.195 1.207 1.201 1.006  
625 0.802 0.743 0.773 0.578  
312 0.489 0.466 0.478 0.283  
156 0.376 0.359 0.368 0.173  
78 0.258 0.243 0.251 0.056  
0 0.201 0.189 0.195    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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