Rat Collagen alpha-1(III) chain (COL3A1) ELISA kit

Code CSB-EL005740RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P13941
Lead Time 7-14 working days
Abbreviation COL3A1
Target Name collagen, type III, alpha 1
Alias EDS4A, FLJ34534, Ehlers-Danlos syndrome type IV, autosomal dominant|alpha1 (III) collagen|collagen type III alpha 1|collagen, fetal,Collagen alpha-1(3) chain (COL3A1),Collagen alpha-1(III) chain (COL3A1)
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 2201
RGD 71029
MGI 88453
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat COL3A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 93    
Range % 89-97    
1:2 Average % 98    
Range % 94-102    
1:4 Average % 95    
Range % 90-103    
1:8 Average % 89    
Range % 84-93    
Recovery
The recovery of rat COL3A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 84 80-88    
EDTA plasma (n=4) 103 99-107    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
200 2.512 2.667 2.590 2.447    
100 2.100 2.025 2.063 1.920    
50 1.431 1.501 1.466 1.323    
25 0.975 1.004 0.990 0.847    
12.5 0.573 0.542 0.558 0.415    
6.25 0.368 0.386 0.377 0.234    
3.12 0.239 0.237 0.238 0.095    
0 0.146 0.140 0.143      
References
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Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity).
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Fibrillar collagen family
Database Links

KEGG: rno:84032

STRING: 10116.ENSRNOP00000004956

UniGene: Rn.3247

Pathway AGE-RAGE signaling pathway in diabetic complications

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