Rat Cytochrome P450 11B2, mitochondrial(CYP11B2) ELISA kit

Unavailable
Code CSB-EL006391RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P30099
Lead Time 5-7 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation CYP11B2
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name cytochrome P450, family 11, subfamily B, polypeptide 2
Alias ALDOS, CPN2, CYP11B, CYP11BL, P-450C18, P450C18, P450aldo, aldosterone synthase|cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2|mitochondrial cytochrome P450, family 11,
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Steroidogenesis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 43.75 pg/mL-2800 pg/mL
Sensitivity 10.93 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP11B2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 87-102
1:2 Average % 91
Range % 84-95
1:4 Average % 93
Range % 85-96
1:8 Average % 104
Range % 97-108
Recovery
The recovery of rat CYP11B2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 84-96
EDTA plasma (n=4) 96 88-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2800 2.531 2.473 2.502 2.319
1400 1.985 1.946 1.966 1.783
700 1.258 1.292 1.275 1.092
350 0.846 0.817 0.832 0.649
175 0.517 0.531 0.524 0.341
87.5 0.359 0.345 0.352 0.169
43.75 0.280 0.274 0.277 0.094
0 0.181 0.185 0.183  
References
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Function Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.
Subcellular Location Mitochondrion membrane
Protein Families Cytochrome P450 family
Tissue Specificity Adrenal cortex.
Database Links

UniGene: Rn.144549

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