Rat Endothelin-converting enzyme 1(ECE1) ELISA kit

Code CSB-EL007371RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P42893
Lead Time 7-14 working days
Abbreviation ECE1
Target Name endothelin converting enzyme 1
Alias RP3-329E20.1, ECE,
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
HGNC 3146
RGD 620293
MGI 1101357
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ECE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 105
Range % 101-109
1:10 Average % 87
Range % 84-90
1:20 Average % 102
Range % 98-106
1:40 Average % 105
Range % 101-109
Recovery
The recovery of rat ECE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-98
EDTA plasma (n=4) 89 85-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average
100 0.142 0.145 0.144
50 0.239 0.236 0.238
25 0.354 0.360 0.357
12.5 0.547 0.538 0.543
6.25 0.764 0.771 0.768
3.12 1.063 1.100 1.082
1.56 1.421 1.469 1.445
0 2.218 2.200 2.209
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Function Converts big endothelin-1 to endothelin-1.
Subcellular Location Cell membrane, Single-pass type II membrane protein
Protein Families Peptidase M13 family
Tissue Specificity All isoforms are expressed in aortic endothelial cells. Isoform A is also expressed in liver; isoform B in smooth muscle cells and fibroblasts; isoform C in aortic endothelial cells, smooth muscle cells, fibroblasts, liver and lung, and isoform D in smoot
Database Links

STRING: 10116.ENSRNOP00000062928

UniGene: Rn.7000

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