Rat Epoxide hydrolase 1(EPHX1) ELISA kit

Unavailable
Code CSB-EL007734RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07687
Lead Time 5-7 working days
Abbreviation EPHX1
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name epoxide hydrolase 1, microsomal (xenobiotic)
Alias EPHX, EPOX, MEH, epoxide hydratase|epoxide hydrolase 1
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Aromatic hydrocarbons catabolism
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 3401
RGD 2557
MGI 95405
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat EPHX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-94
1:2 Average % 102
Range % 97-107
1:4 Average % 93
Range % 88-97
1:8 Average % 96
Range % 92-101
Recovery
The recovery of rat EPHX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 99-108
EDTA plasma (n=4) 87 83-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 1.994 2.144 2.069 1.876
500 1.743 1.832 1.788 1.595
250 1.331 1.373 1.352 1.159
125 0.893 0.898 0.896 0.703
62.5 0.545 0.554 0.550 0.357
31.25 0.378 0.386 0.382 0.189
15.6 0.281 0.289 0.285 0.092
0 0.192 0.193 0.193  
References
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Function Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. May play a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids.
Subcellular Location Microsome membrane, Single-pass type III membrane protein, Endoplasmic reticulum membrane, Single-pass type III membrane protein
Protein Families Peptidase S33 family
Database Links

KEGG: rno:25315

STRING: 10116.ENSRNOP00000004780

UniGene: Rn.3603

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