Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit

Code CSB-E15955r
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P70492
Lead Time 3-5 working days
Abbreviation FGF10
Target Name fibroblast growth factor 10
Alias , keratinocyte growth factor 2|produced by fibroblasts of urinary bladder lamina propria
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Target Details This protein is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing.
HGNC 3666
RGD 2606
MGI 1099809
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat FGF10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 87-104
1:2 Average % 105
Range % 98-111
1:4 Average % 90
Range % 83-95
1:8 Average % 94
Range % 87-98
Recovery
The recovery of rat FGF10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 82-91
EDTA plasma (n=4) 90 81-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.752 2.645 2.699 2.561
500 2.148 2.089 2.119 1.981
250 1.400 1.347 1.374 1.236
125 0.822 0.800 0.811 0.673
62.5 0.566 0.543 0.555 0.417
31.2 0.362 0.354 0.358 0.220
15.6 0.229 0.222 0.226 0.088
0 0.140 0.136 0.138  
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Function Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing.
Subcellular Location Secreted
Protein Families Heparin-binding growth factors family
Tissue Specificity Preferentially expressed in the lung in adults.
Database Links

KEGG: rno:25443

STRING: 10116.ENSRNOP00000016485

UniGene: Rn.44439

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
Regulation of actin cytoskeleton
Ras signaling pathway
Rap1 signaling pathway

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