Rat Glutamate dehydrogenase;GDH/GLDH ELISA Kit

Instructions
Code CSB-E13841r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name glutamate dehydrogenase 1
Alternative Names Glud1 ELISA Kit; GludGlutamate dehydrogenase 1 ELISA Kit; mitochondrial ELISA Kit; GDH 1 ELISA Kit; EC 1.4.1.3 ELISA Kit; Memory-related gene 2 protein ELISA Kit; MRG-2 ELISA Kit
Abbreviation GLUD1
Uniprot No. P10860
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 94 μU/mL-6000 μU/mL
Sensitivity 23.5 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GDH/GLDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 94    
Range % 88-97    
1:2 Average % 107    
Range % 100-111    
1:4 Average % 100    
Range % 89-104    
1:8 Average % 98    
Range % 90-102    
Recovery
The recovery of rat GDH/GLDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 90 83-94    
EDTA plasma (n=4) 108 98-113    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
uU/ml OD1 OD2 Average Corrected    
6000 2.581 2.394 2.488 2.310    
3000 1.980 1.951 1.966 1.788    
1500 1.181 1.134 1.158 0.980    
750 0.703 0.733 0.718 0.540    
375 0.407 0.418 0.413 0.235    
187.5 0.289 0.300 0.295 0.117    
94 0.241 0.233 0.237 0.059    
0 0.180 0.176 0.178      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (By similarity). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
Gene References into Functions
  1. Findings demonstrate that hippocampal synapse assembly and maintenance require a tripartite molecular complex in which the ligand Cbln2 binds with presynaptic neurexin 1beta (+S4) and postsynaptic GluD1. PMID: 29784783
  2. Glutamate dehydrogenase is differentially regulated in liver and muscle of rats with transplanted Guerin's carcinoma. PMID: 24319738
  3. We find that SIRT4 and GDH1 overexpression play antagonistic roles in regulating gliogenesis PMID: 23281078
  4. GDH activity is involved in activation of insulin secretion. PMID: 20332361
  5. GDH1 facilitates regeneration of BCATm that binds to E1 decarboxylase of the branched-chain alpha-keto acid dehydrogenase enzyme complex, promotes metabolon formation, branched-chain amino acid oxidation, and cycling of nitrogen through glutamate. PMID: 19858196
  6. during acidosis, the pH-responsive stabilization of glutamate dehydrogenase mRNA may be accomplished by the same mechanism that affects an increase in mitochondrial glutaminase mRNA PMID: 12684230
  7. inhibition of glutamate dehydrogenase results in sulfite neurotoxicity PMID: 15273247

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Subcellular Location Mitochondrion matrix
Protein Families Glu/Leu/Phe/Val dehydrogenases family
Tissue Specificity Widely expressed throughout the hippocampus. After induction by training, highly expressed in the dentate gyrus, pyrimidal layer and lacunosum molecolare.
Database Links

KEGG: rno:24399

STRING: 10116.ENSRNOP00000013789

UniGene: Rn.55106

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