Rat Gonadotropin-releasing hormone receptor,GnRHR ELISA Kit

Instructions
Code CSB-E11382r
Size 96T,5×96T,10×96T
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Product Details

Target Name gonadotropin-releasing hormone receptor
Alternative Names Gnrhr ELISA Kit; Gonadotropin-releasing hormone receptor ELISA Kit; GnRH receptor ELISA Kit; GnRH-R ELISA Kit
Abbreviation GNRHR
Uniprot No. P30969
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 80 pg/mL-2000 pg/mL
Sensitivity 50 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GnRHR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %86-103
1:2Average %91
Range %85-98
1:4Average %95
Range %87-102
1:8Average %93
Range %80-105
Recovery
The recovery of rat GnRHR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9385-100
EDTA plasma (n=4)9586-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
20000.137 0.135 0.136
8000.234 0.226 0.230
4800.331 0.320 0.326
2000.592 0.627 0.610
801.264 1.238 1.251
02.614 2.685 2.650
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system.
Gene References into Functions
  1. the age-dependent basal and regulated Gnrhr transcription could account for the initial blockade and subsequent activation of the reproductive system during development. PMID: 27569529
  2. Prevention of estradiol synthesis blocked the ability of peripheral estradiol administration in ovariectomized rats to enhance hippocampus-dependent memory in a radial-maze task. Hippocampal GnRH receptor antagonism blocked the ability of peripheral estradiol administration in ovariectomized rats to enhance hippocampus-dependent memory. PMID: 28032117
  3. GnRH-R is expressed in the mammary tissues after weaning; GnRH is involved in the involution and tissue remodeling of post-lactating rat mammary tissues PMID: 27349532
  4. Gnrhr promoter sequences are mainly responsible for directing transcriptional programmes and play a predominant role over the species-specific cell environment. PMID: 25556311
  5. Data indicate that GnRH-R expression is strongest in the hippocampus of adult female rats. PMID: 23906992
  6. Rather than using alternate promoters, Gnrhr expression is directed to diverse cell lineages through specific associations of transcription factors acting on distinct response elements along the same promoter. PMID: 22414758
  7. SET protein interacts with intracellular domains of the gonadotropin-releasing hormone receptor and differentially regulates receptor signaling to cAMP and calcium in gonadotrope cells PMID: 23233674
  8. Data demonstrate herein that the onset of GnRHR gene (Gnrhr) expression in the rat hippocampus was unexpectedly delayed as compared to the pituitary and only occurred after birth. PMID: 21123436
  9. Low-level GnRHR expression in the ovaries of rats with cyclophosphamide exposure suggest microenvironment disturbances in the damaged rat ovaries in advanced stage of chemotherapy. PMID: 18024297
  10. Localization of a functional GHRH-R in Henle's loop and its regulation during development and aging suggest roles associated with cellular proliferation, differentiation, and/or water/electrolyte transport PMID: 11897706
  11. Data show that removal of all three carboxyl terminal residues or mutation of the gonadotropin-releasing hormone receptor at Phe(325) caused a complete loss of measurable ligand binding and effector coupling, clearly suggesting a role for Phe(325). PMID: 11997175
  12. results indicate that the GnRH receptor activates both G(q) and G(s) signaling to regulate gene expression in L beta T2 cells PMID: 12050161
  13. role of the GnRHR carboxyl-terminus in the regulation of the GnRHR by RGS (regulators of G protein signaling) proteins PMID: 12062898
  14. The distribution of androgen receptor and its coexistence with gonadotropin releasing hormone receptor in rat submaxillary glands PMID: 12479044
  15. GnRH receptor immunoreactive materials were colocalized in the epithelial cells of the serous acinus and glandular duct of the submaxillary gland PMID: 12697272
  16. Position of Pro and Ser near Glu7.32 in the extracellular loop 3 is critical for the differential ligand selectivity between mammalian and nonmammalian GnRHRs. PMID: 14525953
  17. direct role for the GnRHR in mediating antiproliferative events in two cell systems, neither of which was derived from extrapituitary reproductive tumors. PMID: 14551223
  18. Developmental GnRH-R and gonadotropin beta subunit gene expression is almost entirely GnRH dependent, not only in the juvenile prepubertal stage but also during the infantile period. PMID: 14561652
  19. GnRH receptor can be expressed by gastric smooth muscle cells of rats. PMID: 15188505
  20. the HFRK motif in the membrane-proximal region confers the differential signal transduction pathways between mammalian and nonmammalian GnRHRs PMID: 15563546
  21. there are species-specific dominant negative receptor trafficking effects of the GnRHR between human, rat and mouse PMID: 15886197
  22. GnRH-R mRNA in the pituitary and hypothalamus of long-term ovariectomized (OVX) rats, at different times of day. PMID: 16672174
  23. proportion of the human and the rat WT GnRHR appears to be retained in the endoplasmic reticulum by calnexin, an effect that decreases GnRHR signaling capacity PMID: 17170088
  24. These results, for the first time, support the transcription of GnRHR mRNA and its translation to protein in the rat oviduct throughout the postimplantation period of pregnancy. PMID: 17875654
  25. Results indicate that the residues flanking Glu(7.32) of the mammalian type-I gonadotropin-releasing hormone receptor are important for antagonist as well as agonist selectivity. PMID: 18319619
  26. Both cultured spinal cord neurons of rat embryos and adult rats expressed the GnRH receptor mRNA. GnRH may play other roles independently from its participation in reproductive function. PMID: 19539704

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family
Database Links

KEGG: rno:81668

STRING: 10116.ENSRNOP00000002755

UniGene: Rn.112595

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