Rat Growth hormone receptor(GHR) ELISA kit

Instructions
Code CSB-EL009411RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth hormone receptor
Alternative Names GhrGrowth hormone receptor ELISA Kit; GH receptor ELISA Kit; Somatotropin receptor) [Cleaved into: Growth hormone-binding protein ELISA Kit; GH-binding protein ELISA Kit; GHBP ELISA Kit; Serum-binding protein)] ELISA Kit
Abbreviation GHR
Uniprot No. P16310
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.44 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GHR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 80-89
1:2 Average % 97
Range % 92-101
1:4 Average % 92
Range % 88-96
1:8 Average % 100
Range % 95-108
Recovery
The recovery of rat GHR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-92
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
6000 2.489 2.335 2.412 2.226
3000 1.925 1.827 1.876 1.690
1500 1.213 1.290 1.252 1.066
750 0.750 0.774 0.762 0.576
375 0.432 0.419 0.426 0.240
187.5 0.316 0.301 0.309 0.123
93.75 0.250 0.263 0.257 0.071
0 0.183 0.188 0.186  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway.; FUNCTION
Gene References into Functions
  1. receptor crosstalk between growth hormone and insulin signal in rats born small for gestational age with catch-up growth PMID: 24963636
  2. The growth hormone receptor expression in the brain was lower during fetal period than in adults, while in the pituitary gland, the expression is markedly higher in fetuses, suggesting a yet unknown role of growth hormone in the development of this organ. PMID: 21177131
  3. Growth hormone receptor mRNA levels decrease in hypothalamus during normal pregnancy while growth hormone expression rises in the pituitary. PMID: 20814072
  4. Data show that TrCP acts in an early stage of cargo selection: both TrCP silencing and mutation of the ubiquitin-dependent endocytosis motif force the GHR to recycle between endosomes and the plasma membrane. PMID: 21195069
  5. GH and its receptor have roles in injury-induced neurogenesis PMID: 19524466
  6. gene expression of GH and GHR mRNA in isolated oocytes and follicular wall cells of pre-antral follicles; localisation of immunoreactive IGF-I, IGFR, GH and GHR proteins in ovarian sections of 10-day-old rats PMID: 11964096
  7. localization in growth plate PMID: 12162495
  8. exerts divergent effects on STAT5-mediated transcription depending on its cellular location PMID: 12488452
  9. findings demonstrate the interaction of cytokine-induced Src homology domain 2 protein(CIS) with the growth hormone receptor in vivo and suggest that CIS may enhance degradation of the receptor by a proteasomal pathway PMID: 12586763
  10. data suggest that muscle atrophy is associated with a muscle fiber type-specific growth hormone receptor mRNA up-regulation mechanism PMID: 12865352
  11. The growth plate distribution of IGF-IR and GHR mRNA implies distinct roles for circulating IGF-I vs. paracrine produced IGF-I. PMID: 15609625
  12. GH receptor in visceral sensory and motor structures is consonant with a role of GH in the regulation of food intake and energy homeostasis. Its presence in the spinal dorsal horn indicates a role for GH in the initial processing of fine afferent input. PMID: 15664698
  13. The growth hormone(GH)-responsive rat liver cell line CWSV-1 was used to investigate the role of CIS and the proteasome in GH-induced GHR internalization. PMID: 16154995
  14. In the intrauterine growth restricted rat at one year of age, levels of pituitary GH, GH-R and GHRH-R relative gene expression were increased. PMID: 16867182
  15. acetaminophen indirectly stimulates spinal (5-HT)1A receptors, thereby increasing transcript and protein levels of low-affinity neurotrophin receptor,(IGF-1) receptor alpha subunit, and growth hormone receptor and reducing the amount of sst3R mRNA PMID: 17088403
  16. results suggest that the acquisition of mechanisms responsible for the regulation of GH or GHRH-receptor transcription by T3 may be involved in the process of functional development of GH cells PMID: 17090972
  17. These findings indicate that the heterologous reduction of GHR by insulin occurs via transcriptional downregulation, and the homologous reduction of GHR by GH occurs via a different mechanism. PMID: 17658679
  18. Insulin signaling via either the PI-3 kinase or MEK/ERK pathway may result in partial reduction of GHR gene expression, whereas signaling via both pathways may be required to achieve the full insulin effect. PMID: 18040895

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Subcellular Location Cell membrane, Single-pass type I membrane protein, Note=On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway, SUBCELLULAR LOCATION: Isoform 2: Secreted, Membrane, Note=Mainly secreted, In adipose tissue, isoform 2 is mostly membrane associated, SUBCELLULAR LOCATION: Growth hormone-binding protein: Secreted
Protein Families Type I cytokine receptor family, Type 1 subfamily
Tissue Specificity Highest expression in liver. Also expressed in heart, kidney and muscle.
Database Links

KEGG: rno:25235

STRING: 10116.ENSRNOP00000044119

UniGene: Rn.2178

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