Rat Hormone-sensitive lipase(LIPE) ELISA kit

Instructions
Code CSB-EL012975RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lipase, hormone-sensitive
Alternative Names Lipe ELISA Kit; Hormone-sensitive lipase ELISA Kit; HSL ELISA Kit; EC 3.1.1.79 ELISA Kit
Abbreviation LIPE
Uniprot No. P15304
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 mU/mL-1000 mU/mL
Sensitivity 3.9 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LIPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 88-96
1:2 Average % 95
Range % 90-102
1:4 Average % 97
Range % 94-104
1:8 Average % 101
Range % 95-105
Recovery
The recovery of rat LIPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-96
EDTA plasma (n=4) 98 95-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
1000 2.184 2.210 2.197 2.013
500 1.486 1.530 1.508 1.324
250 0.925 0.965 0.945 0.761
125 0.545 0.529 0.537 0.353
62.5 0.431 0.422 0.427 0.243
31.2 0.308 0.321 0.315 0.131
15.6 0.251 0.250 0.251 0.067
0 0.179 0.189 0.184  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Gene References into Functions
  1. Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes, HSL and ATGL, in rat primary white adipocytes. PMID: 26141235
  2. The findings suggest that progesterone, acting in a progesterone receptor-dependent manner, down-regulates Lipe gene expression but exerts no effect on Pnpla2 gene expression in the inguinal white adipose tissue of females. PMID: 25448749
  3. These findings suggest that acute exercise enhances lipolysis up to at least 3h after exercise in a manner dependent on modification of HSL and its association with and alteration in scaffold protein. PMID: 20708600
  4. When compared with obesity-resistant rats, the gene expression of HSL in white adipose tissue was lower in obesity-prone rats. PMID: 17712951
  5. hormone-sensitive lipase is involved in mediating beta-cell lipotoxicity by providing ligands for peroxisome proliferator-activated receptors and other lipid-activated transcription factors, which in turn alter the expression of critical genes. PMID: 12882923
  6. interaction of HSL with StAR in cytosol increases the hydrolytic activity of HSL and that together HSL and steroidogeneic acute regulatory protein facilitate cholesterol movement from lipid droplets to mitochondria for steroidogenesis PMID: 12925534
  7. an 89-kDa isoform of hormone-sensitive lipase (HSL) and its molecular structure is described; the novel HSL isoform is expressed in the pancreatic beta-cell, adipose tissue, adrenal gland, and ovary PMID: 14576146
  8. hormone sensitive lipase is phosphorylated by 5' AMP-activated protein kinase at Ser(565) during muscle contractions, but this phosphorylation is not directly responsible for the contraction-induced activation of HSL PMID: 15033481
  9. Immunoreactive hormone-sensitive lipase (HSL) and HSL activity were found in lactating rats' milk PMID: 15654127
  10. The length of the regulatory module and specific sequences within the C-terminal portion of the regulatory module of hormone sensitive lipase (amino acids 644-683) are crucial for activity and appear to be responsible for determining lipase activity. PMID: 15697220
  11. Upregulated by rosiglitazone. PMID: 15887043
  12. HSL labeling in the nucleus of epithelial and germ cells suggests an as yet unknown function for this protein, probably related to oogenesis and cell proliferation. PMID: 18824635
  13. The retinyl ester hydrolase activity of rat HSL was found to be higher than that against the hitherto best known HSL substrate, i.e., diacylglycerols. PMID: 19246492
  14. PKA phosphorylation induces a conformational change that increases the exposed hydrophobic surface and thereby facilitates binding of HSL to the lipid substrate. PMID: 19664063

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Subcellular Location Cell membrane, Membrane, caveola, Cytoplasm, cytosol
Protein Families 'GDXG' lipolytic enzyme family
Database Links

KEGG: rno:25330

STRING: 10116.ENSRNOP00000027911

UniGene: Rn.10566

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