Rat Inhibitor of nuclear factor kappa-B kinase subunit beta(IKBKB) ELISA kit

Code CSB-EL011572RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Alternative Names Ikbkb ELISA Kit; IkkbInhibitor of nuclear factor kappa-B kinase subunit beta ELISA Kit; I-kappa-B-kinase beta ELISA Kit; IKK-B ELISA Kit; IKK-beta ELISA Kit; IkBKB ELISA Kit; EC ELISA Kit; I-kappa-B kinase 2 ELISA Kit; IKK2 ELISA Kit; Nuclear factor NF-kappa-B inhibitor kinase beta ELISA Kit; NFKBIKB ELISA Kit
Abbreviation IKBKB
Uniprot No. Q9QY78
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat IKBKB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 104
Range % 100-108
1:2 Average % 92
Range % 88-97
1:4 Average % 84
Range % 80-88
1:8 Average % 98
Range % 94-102
The recovery of rat IKBKB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 89 84-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.238 2.182 2.210 2.115
1000 1.526 1.495 1.511 1.416
500 0.892 0.874 0.883 0.788
250 0.541 0.511 0.526 0.431
125 0.304 0.318 0.311 0.216
62.5 0.237 0.226 0.232 0.137
31.25 0.162 0.159 0.161 0.066
0 0.093 0.097 0.095  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Gene References into Functions
  1. Inhibition of IkappaB kinase at peak creatinine in acute kidny injury improves functional recovery, reduces further injury, and prevents fibrosis. PMID: 28673900
  2. Data indicate that injury-induced upregulation of inflammatory cytokines within IVDs and increased levels of neuropeptides within DRG neurons can be suppressed by inhibiting IkappaB kinase-beta (IKKbeta). PMID: 24825147
  3. IKKbeta could reduce arterial remodeling events by affecting both the inflammatory and the growth-promoting response of Ang II. PMID: 24135021
  4. Selective inhibition of IkappaBbeta-mediated NF-kappaB activation maintains the inflammatory stress-induced upregulation of antioxidant enzyme and preserves cellular growth, as shown in fetal pulmonary vascular endothelial cells. PMID: 23418625
  5. Following treatment with polymixin B, I-kappaB kinase mRNA expression was downregulated in pulmonary alveolar macrophages. PMID: 12857501
  6. down-regulation of the NF-kappaB pathway via IKKbeta inhibition dampened the chronic inflammatory process associated with rat adjuvant-induced arthritis [IKKbeta] PMID: 17009244
  7. With the prolongation of smoking duration, the phosphorylation of IKK-ss in type 2 diabetic rats increased. After smoking cessation, the phosphorylation of IKK-ss decreased. PMID: 20646420
  8. Report the IkappaB kinase inhibitor nuclear factorkappaB essential modulator-binding domain peptide inhibits injury-induced neointimal formation. PMID: 20930169
  9. data suggest that NGF-induced PC12 differentiation includes activation of IKK2 which may promote the release of c-Rel-containing dimers PMID: 15935065
  10. canonical IKKbeta-dependent NF-kappaB activation is inhibited by peroxynitrite, which also activates NF-kappaB-inducing kinase and IKKalpha phosphorylation PMID: 16079150
  11. dominant negative IKK2-Treg is a unique population of CD4CD25 T cells expressing high levels of inducible nitric oxide synthase. PMID: 17318081
  12. both IKKalpha and IKKbeta are necessary and sufficient for physiological muscle atrophy PMID: 18827022
  13. differences in glucose tolerance between rats with a low capacity for running and rats with a high capacity to run are at least partly explained by differences in IKKbeta activity and pSer (307) levels PMID: 19569009

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Subcellular Location Cytoplasm, Nucleus, Membrane raft
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
Database Links

KEGG: rno:84351

STRING: 10116.ENSRNOP00000025851

UniGene: Rn.19222


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