Name: Rat Inhibitor of nuclear factor kappa-B kinase subunit beta(IKBKB) ELISA kit
Brand: CUSABIO
SKU: CSB-EL011572RA

Product Details

Target Name

inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta

Alternative Names

Ikbkb ELISA Kit; IkkbInhibitor of nuclear factor kappa-B kinase subunit beta ELISA Kit; I-kappa-B-kinase beta ELISA Kit; IKK-B ELISA Kit; IKK-beta ELISA Kit; IkBKB ELISA Kit; EC 2.7.11.10 ELISA Kit; I-kappa-B kinase 2 ELISA Kit; IKK2 ELISA Kit; Nuclear factor NF-kappa-B inhibitor kinase beta ELISA Kit; NFKBIKB ELISA Kit

Abbreviation

IKBKB

Uniprot No.

Q9QY78

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IKBKB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-108
1:2	Average %	92
	Range %	88-97
1:4	Average %	84
	Range %	80-88
1:8	Average %	98
	Range %	94-102

Recovery

The recovery of rat IKBKB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.238	2.182	2.210	2.115
1000	1.526	1.495	1.511	1.416
500	0.892	0.874	0.883	0.788
250	0.541	0.511	0.526	0.431
125	0.304	0.318	0.311	0.216
62.5	0.237	0.226	0.232	0.137
31.25	0.162	0.159	0.161	0.066
0	0.093	0.097	0.095

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

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Target Data

Function	Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
Gene References into Functions	Inhibition of IkappaB kinase at peak creatinine in acute kidny injury improves functional recovery, reduces further injury, and prevents fibrosis. PMID: 28673900 Data indicate that injury-induced upregulation of inflammatory cytokines within IVDs and increased levels of neuropeptides within DRG neurons can be suppressed by inhibiting IkappaB kinase-beta (IKKbeta). PMID: 24825147 IKKbeta could reduce arterial remodeling events by affecting both the inflammatory and the growth-promoting response of Ang II. PMID: 24135021 Selective inhibition of IkappaBbeta-mediated NF-kappaB activation maintains the inflammatory stress-induced upregulation of antioxidant enzyme and preserves cellular growth, as shown in fetal pulmonary vascular endothelial cells. PMID: 23418625 Following treatment with polymixin B, I-kappaB kinase mRNA expression was downregulated in pulmonary alveolar macrophages. PMID: 12857501 down-regulation of the NF-kappaB pathway via IKKbeta inhibition dampened the chronic inflammatory process associated with rat adjuvant-induced arthritis [IKKbeta] PMID: 17009244 With the prolongation of smoking duration, the phosphorylation of IKK-ss in type 2 diabetic rats increased. After smoking cessation, the phosphorylation of IKK-ss decreased. PMID: 20646420 Report the IkappaB kinase inhibitor nuclear factorkappaB essential modulator-binding domain peptide inhibits injury-induced neointimal formation. PMID: 20930169 data suggest that NGF-induced PC12 differentiation includes activation of IKK2 which may promote the release of c-Rel-containing dimers PMID: 15935065 canonical IKKbeta-dependent NF-kappaB activation is inhibited by peroxynitrite, which also activates NF-kappaB-inducing kinase and IKKalpha phosphorylation PMID: 16079150 dominant negative IKK2-Treg is a unique population of CD4CD25 T cells expressing high levels of inducible nitric oxide synthase. PMID: 17318081 both IKKalpha and IKKbeta are necessary and sufficient for physiological muscle atrophy PMID: 18827022 differences in glucose tolerance between rats with a low capacity for running and rats with a high capacity to run are at least partly explained by differences in IKKbeta activity and pSer (307) levels PMID: 19569009 Show More Hide All
Subcellular Location	Cytoplasm, Nucleus, Membrane raft
Protein Families	Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
Database Links	KEGG: rno:84351 STRING: 10116.ENSRNOP00000025851 UniGene: Rn.19222

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Check Code：

Code	CSB-EL011572RA
Size	96T,5×96T,10×96T
Trial Size
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Rat Inhibitor of nuclear factor kappa-B kinase subunit beta(IKBKB) ELISA kit

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