Rat Interferon β,IFN-β/IFNB ELISA Kit

Code CSB-E04845r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
interferon, beta 1, fibroblast
Alternative Names
Ifnb1 ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
Abbreviation
IFNB1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %94-102
1:2Average %104
Range %98-110
1:4Average %90
Range %85-94
1:8Average %93
Range %86-98
Recovery
The recovery of pig LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)8780-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.516 2.587 2.552 2.380
5002.021 2.106 2.064 1.892
2501.362 1.324 1.343 1.171
1250.761 0.739 0.750 0.578
62.50.503 0.534 0.519 0.347
31.250.375 0.359 0.367 0.195
15.60.276 0.295 0.286 0.114
00.174 0.170 0.172
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat IFNB1 ELISA Kit was designed for the quantitative measurement of Rat IFNB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

 Q&A
Q:

I will order IFN beta from you,
https://www.cusabio.com/Recombinant-Protein/Recombinant-Rat-Interferon-betaIfnb1-189.html
Could you guarantee I could measure that rat IFN beta with CSB-E04845r ELISA kit?
Is the two item compatible?

A:
Thanks for your inquiry.
The standard of this kit CSB-E04845r is NS0-derived recombinant protein.
The recombinant proteins with different expression systems will be different. Sorry we didn't validate the detailed differences before, so we can not 100% guarantee that the kit could measure the protein.We suggest you do a pretest first.

Target Background

Function
(From Uniprot)
Has antiviral, antibacterial and anticancer activities.
Gene References into Functions
  1. results provided evidence that delivery of IL-18 and IFN-beta by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy. PMID: 26252165
  2. IFN-gamma is able to activate rat intestinal mucous microvascular endothelial cells, thereby leading to upregulated expressions of IFN-alpha/beta. PMID: 20214528
  3. These findings validate IFN-beta as a therapeutic molecule for intraarticular gene therapy of arthritis. PMID: 16984225
  4. This study presents novel effects of IFN-beta in the central nervous system and begins to unravel the mechanism behind the known excitability changes in neurons. PMID: 19541371
Subcellular Location
Secreted.
Protein Families
Alpha/beta interferon family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1