Rat Interferon β,IFN-β/IFNB ELISA Kit

Instructions
Code CSB-E04845r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interferon, beta 1, fibroblast
Alternative Names Ifnb1 ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
Abbreviation IFNB1
Uniprot No. P70499
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %94-102
1:2Average %104
Range %98-110
1:4Average %90
Range %85-94
1:8Average %93
Range %86-98
Recovery
The recovery of pig LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)8780-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.516 2.587 2.552 2.380
5002.021 2.106 2.064 1.892
2501.362 1.324 1.343 1.171
1250.761 0.739 0.750 0.578
62.50.503 0.534 0.519 0.347
31.250.375 0.359 0.367 0.195
15.60.276 0.295 0.286 0.114
00.174 0.170 0.172
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

I will order IFN beta from you,
https://www.cusabio.com/Recombinant-Protein/Recombinant-Rat-Interferon-betaIfnb1-189.html
Could you guarantee I could measure that rat IFN beta with CSB-E04845r ELISA kit?
Is the two item compatible?

A:
Thanks for your inquiry.
The standard of this kit CSB-E04845r is NS0-derived recombinant protein.
The recombinant proteins with different expression systems will be different. Sorry we didn't validate the detailed differences before, so we can not 100% guarantee that the kit could measure the protein.We suggest you do a pretest first.

Target Background

Function
(From Uniprot)
Has antiviral, antibacterial and anticancer activities.
Gene References into Functions
  1. results provided evidence that delivery of IL-18 and IFN-beta by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy. PMID: 26252165
  2. IFN-gamma is able to activate rat intestinal mucous microvascular endothelial cells, thereby leading to upregulated expressions of IFN-alpha/beta. PMID: 20214528
  3. These findings validate IFN-beta as a therapeutic molecule for intraarticular gene therapy of arthritis. PMID: 16984225
  4. This study presents novel effects of IFN-beta in the central nervous system and begins to unravel the mechanism behind the known excitability changes in neurons. PMID: 19541371
Subcellular Location Secreted.
Protein Families Alpha/beta interferon family
Database Links

KEGG: rno:24481

STRING: 10116.ENSRNOP00000008225

UniGene: Rn.138105

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