Rat Kynurenine 3-monooxygenase(KMO) ELISA kit

Instructions
Code CSB-EL012475RA
Size 96T,5×96T,10×96T
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Product Details

Target Name kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Alternative Names Kmo ELISA Kit; Kynurenine 3-monooxygenase ELISA Kit; EC 1.14.13.9 ELISA Kit; Kynurenine 3-hydroxylase ELISA Kit
Abbreviation KMO
Uniprot No. O88867
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat KMO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 86-95
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of rat KMO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.586 2.687 2.637 2.514
500 1.984 1.824 1.904 1.781
250 1.271 1.297 1.284 1.161
125 0.842 0.854 0.848 0.725
62.5 0.508 0.521 0.515 0.392
31.2 0.336 0.322 0.329 0.206
15.6 0.233 0.237 0.235 0.112
0 0.122 0.123 0.123  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.
Subcellular Location Mitochondrion outer membrane, Multi-pass membrane protein
Protein Families Aromatic-ring hydroxylase family, KMO subfamily
Tissue Specificity Highest activity in liver and kidney. Low activity in spleen, stomach, intestinal tract, esophagus, heart and lung.
Database Links

KEGG: rno:59113

STRING: 10116.ENSRNOP00000005005

UniGene: Rn.35029

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