Rat Leptin Soluble Receptor,sLR ELISA Kit

Instructions
Code CSB-E04842r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name leptin receptor
Alternative Names Lepr ELISA Kit; Fa ELISA Kit; Obr ELISA Kit; Leptin receptor ELISA Kit; LEP-R ELISA Kit; OB receptor ELISA Kit; OB-R ELISA Kit; CD antigen CD295 ELISA Kit
Abbreviation LEPR
Uniprot No. Q62959
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.9 pg/mL-250 pg/mL
Sensitivity 1 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 97
Range % 92-102
1:400 Average % 91
Range % 84-97
1:800 Average % 90
Range % 87-94
1:1600 Average % 100
Range % 95-103
Recovery
The recovery of rat sLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-93
EDTA plasma (n=4) 106 102-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
250 2.117 2.212 2.165 2.061
125 1.703 1.844 1.774 1.670
62.5 1.219 1.312 1.266 1.162
31.25 0.846 0.803 0.825 0.721
15.6 0.493 0.501 0.497 0.393
7.8 0.338 0.340 0.339 0.235
3.9 0.217 0.219 0.218 0.114
0 0.105 0.102 0.104  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones
Gene References into Functions
  1. Leptin receptor was detected sciatic nerve PMID: 30339059
  2. These results suggest that leptin and its receptor may serve significant roles in the pathogenesis of varicocele-induced testicular dysfunction. PMID: 29568885
  3. high gonadal and gonadal-white adipose tissue expression of leptin system was observed in the offspring of dams fed a high fat diet during pregnancy and lactation. PMID: 28759941
  4. This study showed that the maternal undernutrition rats, the 24h food deprivation group displayed significantly higher hypothalamic leptin receptor (OBRb) mRNA levels than the Fed group PMID: 26702900
  5. Results demonstrate that cardiac mitochondria express functional leptin receptors suggesting that intracellularly derived leptin functions as an autocrine factor acting on both cell membrane and mitochondrial receptors. PMID: 26122392
  6. LEPR is expressed at the cell surface of atrial, ventricular, and sinus node cardiomyocytes. PMID: 26408544
  7. Hipocampal lepr expression was lower in experimental diabetic group than control group. learning performance of the control group was found to be better than the diabetic group. Lepr gene may be related to learning performance in diabetic rats. PMID: 25380550
  8. Carotid chemoreceptor afferent projections to leptin receptor containing neurons in nucleus of the solitary tract. PMID: 24905621
  9. There is an interrelationship between cannabinoid-1 receptor and ObR pathways. PMID: 24903921
  10. The influence of the Lepr(fa/fa) mutation on urinary albumin excretion quantitative trait loci unlinked to LEPR, were identified. PMID: 20159938
  11. reduction of leptin up-regulates leptin receptor expression and may affect prefrontal cortex neurons PMID: 24007473
  12. NPY and its NPY Y1 receptor play a critical role in reproduction by modulating leptin receptor expression. PMID: 24291064
  13. Direct leptin activation of nucleus of the solitary tract slightly increases UCP1 levels, but has little effect on food consumption or body weight. PMID: 24920667
  14. Genistein stimulates fatty acid oxidation in a leptin receptor-independent manner through the JAK2-mediated phosphorylation and activation of AMPK in skeletal muscle. PMID: 24013029
  15. Our results provide evidence that Lrb knockdown selectively in the ARC promotes diet-induced obesity and associated metabolic complications in rats. PMID: 23549406
  16. Data suggest leptin/Lepr signal transduction in ventromedial hypothalamic nucleus mediates glucose homeostasis; intraventricular leptin infusion normalizes diabetic hyperglycemia, but this action may not require Lepr. PMID: 23782941
  17. Changes are examined in leptin sensitivity by leptin resistance-induced leptin receptor (ObRb) and leptin-related suppressor of cytokine signaling 3 (SOCS3) mRNA levels after 8 weeks of exercise training and/or dietary control of high fat induced obesity PMID: 23669042
  18. Data suggest that impairment of leptin/leptin receptor signaling in early life promotes leptin/insulin resistance, modifies hypothalamic microRNA expression pattern, and leads to overweight in adulthood. PMID: 23576026
  19. Leptin receptor interacts with rat chromosome 1 to regulate renal disease traits PMID: 22968639
  20. Congenic rat strain carrying single QTL derived from the OLETF rat shows severe hyperglycemia when combined with obese phenotype via leptin receptor mutation. PMID: 23304119
  21. Maternal running during pregnancy resulted in significant increase in the expression of leptin receptor in offspring, which improved learning memory capability in prepubertal and adult rat pups. PMID: 23028249
  22. Diet-induced obesity and related metabolic disorders are possibly correlated with up-regulated expression of MCHR1 and down-regulated expression of OB-Rb in adipose tissue. PMID: 21946196
  23. There are three subtypes of Leptin receptors expressed in the male rat anterior pituitary. PMID: 21158112
  24. Hypothyroidism decreased ObRb-STAT3 signalling in the hypothalamus and pituitary gland, which likely contributes to the loss of leptin action on food intake and TSH secretion, as previously observed in hypothyroid rats. PMID: 22875962
  25. The findings strongly supported the hypothesis that endogenous neuronal leptin receptor signaling in the medial nucleus tractus solitarius contributes to the control of meal-related satiation signaling and overall food intake and body weight regulation. PMID: 22693203
  26. The objective of the present study was to investigate the effects of obese type 2 diabetes on bone turnover, bone mass, and bone strength in the Spontaneously Diabetic Torii-Lepr fa rat. PMID: 22038286
  27. The medium chain triglyceride diet could decrease body weight gain by increasing Ob-Rb expression in hypothalamus and decreasing NPY level. PMID: 19877509
  28. The early and delayed ischemia/reperfusion neuronal damage were accompanied with reduction of leptin receptor expression cerebral ischemia. PMID: 21180167
  29. The expression of leptin receptor isoforms was detected in the carotid bodies of type I cells but not type II cells. PMID: 21334312
  30. Different leptin receptor expression patterns might partly contribute to age- and gender- related differences in trabecular bone mass PMID: 21452040
  31. Leptin receptors had a fast response to cold exposure normalizing the lower levels observed in the leptin-programmed animals and may contribute to the higher thyroid hormone cold responses. PMID: 17951546
  32. both endospanins are regulators of postinternalization membrane traffic of the endocytic pathway of OB-R. PMID: 21454707
  33. The development of obesity via long-termfeeding of a high-fat diet to rats does not result in cardiac lipotoxicity but promotes the down-regulation of cardiac leptin receptors PMID: 21457721
  34. LepRb receptors are highly expressed in the solitary nucleus, dorsal motor nucleus of the vagus and catecholaminergic neurons of the ventrolateral medulla. Co-localization of LepRb and TRHR1 receptor is not observed on individual fibers in the hindbrain. PMID: 20691166
  35. The increase of both a and b isoform leptin receptors in prostate ventral lobe is related to development of prostate disease. PMID: 21257164
  36. An interaction between CCKAR and OB-Rb in these neurons likely facilitates leptin mediation of short-term satiety. PMID: 21109591
  37. studies in leptin receptor deficient rats (homozygotes & heterozygotes Zucker rats) suggest leptin receptor is not required for modulation of serum insulin via Mc4r in pancreas & pancreatic (autonomic) neurons PMID: 20963574
  38. Data describe effects of activity-based anorexia on muscle and fat depots by studying ghrelin and leptin receptors. expression. Although PMID: 20600421
  39. these data clearly implicate the nucleus of the solitary tract and the hypothalamic retrochiasmatic area as key sites through which brain leptin impacts skeletal muscle via leptin receptors PMID: 20501326
  40. Obesity and type 2 diabetes in the BioBreeding diabetes-resistant (BBDR) rat result in islet expansion associated with protection from T1D in female but not male BBDR(lepr-/lepr-,Gimap5-/Gimap5-) congenic rats. PMID: 19996157
  41. there was the decreased hypothalamus, liver and skeletal ObRb expression in hyperphagic obese Otsuka Long-Evans Tokushima Fatty rats. PMID: 20171169
  42. In addition to the presence of ObR mRNA, ObR protein was shown in both astrocytes and neurons in the rat hypothalamus. PMID: 19747514
  43. Influence of leptin on neurotransmitter overflow from the rat brain in vitro PMID: 11786145
  44. role in increasing leptin mRNA, gene transcription and promoter activity in obese tats PMID: 11829746
  45. presence of the long leptin receptor isoform in the absorptive cells of the small intestine, suggesting that leptin could have a physiological role in the regulation of nutrient absorption PMID: 12010881
  46. relative leptin resistance in rat pregnancy is due primarily to increased plasma leptin-binding activity rather than to changes in hypothalamic leptin receptor expression PMID: 12021059
  47. Mediates leptin potentiation of thermogenic sympathetic responses to hypothermia PMID: 12145155
  48. expression of leptin receptors in rat osteoblasts PMID: 12297277
  49. Prevention of leptin binding to its receptor suppresses rat leukemic cell growth by inhibiting angiogenesis. PMID: 12393625
  50. Food deprivation enhanced NPY and suppressed leptin receptor expression in the ARN and PVN of the hypothalamus. Nicotine administration resulted in decreased NPY and increased leptin receptor levels. PMID: 12576193
  51. In rat testis, leptin receptor expression is characteristic of mature Leydig cells (fetal and adult), and it is functional in adult but not prepubertal life. PMID: 12606446
  52. results indicate that whereas the leptin receptors in the paraventricular nucleus are involved in the tonic regulation of food intake, they might not be actively involved in the tonic regulation of cardiovascular functions PMID: 12824696
  53. findings indicate that acetate and butyrate enhance leptin expression in bovine, but not in rat anterior pituitary cells while butyrate suppresses leptin receptor expression in both rat and bovine pituitaries PMID: 12928006
  54. Ovarian expression of leptin and its receptor is regulated across the cycle by gonadotropins, with peak expression at ovulation, indicating a possible involvement in oocyte maturation, angiogenesis, follicle rupture, or subsequent corpus luteum formation. PMID: 12959975
  55. examined the skeleton of obese leptin receptor-deficient Zucker rats and found significant increases in osteoclast surface in the obese rats at both 9 and 15 weeks without a change in osteoclast number PMID: 12968669
  56. Data indicate that the neonatal pituitary gland expresses leptin and leptin receptors at levels far in excess of those observed in mature rats. PMID: 14615055
  57. modification of estrogen levels or treatment with a selective estrogen receptor modulator, raloxifene (RAL), alters leptinemia and modulates leptin receptor (Ob-R) abundance in hypothalamus and white adipose tissue PMID: 15059958
  58. Rats overfed during early postnatal life show a leptin-resistant state mediated by down-regulation of s hypothalamic leptin receptor isoform. PMID: 15616803
  59. significant reduction of leptin receptor mRNA levels was observed in the ventromedial hypothalamus during pregnancy compared with the nonpregnant controls PMID: 15905318
  60. Shedding of ObRa membrane-anchored receptors, rather than ObRe expression, might preferentially contribute to generation of increased levels of soluble leptin receptor in serum under conditions of food restriction. PMID: 16037380
  61. Results suggest that changes in leptin level and its receptor expression may influence the energy homeostasis during pregnancy. PMID: 16328017
  62. Circulating leptin does not seem to play a key role in the progression of chronic arthritis. PMID: 16962144
  63. Findings strongly suggest that leptin receptors may be involved in the autocrine-paracrine functional regulation of the epithelial cells of adult rat seminal vesicles and prostate. PMID: 16964413
  64. Both direct and indirect (hypothalamic) leptin receptor-mediated actions of hyperleptinemia are involved in depletion of adipocyte fat. PMID: 17038325
  65. The interaction between genetic predisposition and factors in the maternal environment on the development of hypothalamic peptide systems involved in energy homeostasis regulation was studied. PMID: 17218441
  66. that the leptin receptor system is differentially regulated during late gestation and early postnatal life in the rat PMID: 17391913
  67. The results suggest that a leptin downregulated OB-R expression is one of leptin resistant mechanisms for maintaining obesity. PMID: 17611900
  68. The juvenile rat pituitary might prepare for a subsequent increase of serum leptin concentration by expressing Ob-Rb. PMID: 18250539
  69. The ability of a leptin receptor-neutralizing antibody to improve cardiac function offers evidence that endogenous leptin contributes to cardiac hypertrophy following coronary artery ligation. PMID: 18469142
  70. increased levels of endogenous cannabinoids in the Obese rats corroborating the regulation of cannabinoid signaling by leptin PMID: 18563836
  71. data indicate presence of cardiac leptin resistance in diet-induced obesity possibly associated with impaired leptin receptor signaling. PMID: 18719678
  72. exercise training-induced decrease in plasma leptin levels is accompanied by a reduction in gene expression of three different isoforms of leptin receptors in liver PMID: 19082917
  73. study showed for the first time leptin receptor expression in a subpopulation of glial fibrillary acid protein positive cells located at the border between the area postrema and the nucleus tractus solitarius PMID: 19094094
  74. Postnatal early overnutrition changes the leptin signalling pathway in the hypothalamic-pituitary-thyroid axis of young and adult rats. PMID: 19403617
  75. These results provide direct evidence that the soluble leptin receptor (SLR) neutralizes leptin, endogenous or exogenous, in vivo. By neutralizing leptin, the SLR may play a regulatory role in energy homeostasis PMID: 19422379
  76. Down-regulation of IL-6st and leptin receptor in glia by cytokines could potentially affect the disease course in MS. PMID: 19479985
  77. Maternal diet affects folliculogenesis and leptin and leptin receptors expression in offspring. PMID: 19591986
  78. A direct influence of disrupted leptin signalling on CB(1)R mRNA regulation was demonstrated. PMID: 19596404
  79. Hindbrain leptin receptor stimulation enhances the anorexic response to cholecystokinin. Leptin signaling in either forebrain or hindbrain areas can enhance the response to satiation signals. PMID: 19726710
  80. There is a critical role of unrestrained leptin receptor activity in long-term energy homeostasis and even minor disruption of leptin receptor function can promote obesity. PMID: 19726711

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Involvement in disease The fatty (Fa) mutation produces profound obesity of early onset caused by hyperphagia, defective non-shivering thermogenesis, and preferential deposition of energy into adipose tissue.
Subcellular Location Cell membrane, Single-pass type I membrane protein, Basolateral cell membrane, SUBCELLULAR LOCATION: Isoform E: Secreted
Protein Families Type I cytokine receptor family, Type 2 subfamily
Tissue Specificity Isoform B is expressed in kidney, liver, lung, ovary, spleen and uterus. Increased level in uterus during gestation (PubMed:8772180). Isoform A and isoform C are predominantly expressed in cerebral microvessels and choroid plexus, with lower levels in cor
Database Links

KEGG: rno:24536

STRING: 10116.ENSRNOP00000046647

UniGene: Rn.9891

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