Rat Membrane primary amine oxidase(AOC3) ELISA kit

Instructions
Code CSB-EL001855RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name amine oxidase, copper containing 3 (vascular adhesion protein 1)
Alternative Names Aoc3Membrane primary amine oxidase ELISA kit; EC 1.4.3.21 ELISA kit; Copper amine oxidase ELISA kit; Semicarbazide-sensitive amine oxidase ELISA kit; SSAO ELISA kit; VP97 ELISA kit; Vascular adhesion protein 1 ELISA kit; VAP-1 ELISA kit
Abbreviation AOC3
Uniprot No. O08590
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %84-94
1:2Average %96
Range %91-102
1:4Average %84
Range %81-88
1:8Average %93
Range %88-99
Recovery
The recovery of rat AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-97
EDTA plasma (n=4)9491-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
5002.075 2.086 2.081 1.931
2501.540 1.669 1.605 1.455
1251.111 1.156 1.134 0.984
62.50.758 0.750 0.754 0.604
31.20.437 0.465 0.451 0.301
15.60.261 0.277 0.269 0.119
7.80.211 0.223 0.217 0.067
00.149 0.150 0.150  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis (By similarity).
Gene References into Functions
  1. Data show that 35 potential amine oxidase copper containing 3 (SSAO)-interacting proteins were found to be altered in type 2 Diabetes (T2D). PMID: 28910971
  2. VAP-1 plays a critical role in the pathophysiology of renal ischemia/reperfusion injury by enhancement of neutrophil infiltration generating a local hydrogen peroxide gradient. PMID: 28318627
  3. The changes of K(M) and k(c)/K(M) values of copper amine oxidase appear due to the presence of positively charged groups in the substrate molecules. PMID: 20686801
  4. Provide evidence for a role for VAP-1 in the inflammatory outcome of diabetic retinopathy. Specifically, VAP-1 plays a central role in regulating leukocyte transmigration rate during chronic low-grade inflammation. PMID: 19635478
  5. Our parsimonious conclusion is that there is a relation between the risk factors of AD and vascular dementia (diabetes, oxidative stress and chronic inflammation) and SSAO activity, which may originate from the vessel wall. PMID: 16524643
  6. Enzymatic activity of the atypical endothelial adhesion molecule AOC3, and possibly that of other related oxidases, is crucial for leukocyte exit from the vessels in inflamed joints in vivo. PMID: 16947396
  7. It was concluded that hydroxylamine exerts hypotension partly through conversion to nitric oxide and partly by a "hydralazine-like" mechanism involving SSAO inhibition PMID: 17385063
  8. BZA + V treatment did not reduce HbA(1C) levels,but it reduced the elevated serum SSAO activity, decreased advanced-glycation end products and increased nitric oxide in diabetic animals. PMID: 17431735
  9. evidence for an important role of VAP-1 in the recruitment of macrophages to choroidal neovascularization lesions, establishing a novel link between VAP-1 and angiogenesis PMID: 18436961

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Subcellular Location Membrane, Single-pass type II membrane protein
Protein Families Copper/topaquinone oxidase family
Tissue Specificity Highly expressed in adipocytes, aorta and lung. Expressed at lower levels in heart, kidney, large intestine, liver, small intestine and stomach.
Database Links

KEGG: rno:29473

STRING: 10116.ENSRNOP00000059889

UniGene: Rn.198327

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