Rat Metallothionein-2(MT-2) ELISA Kit

Instructions
Code CSB-E14368r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Metallothionein-2(MT-2)
Alternative Names Mt2 ELISA Kit; Metallothionein-2 ELISA Kit; MT-2 ELISA Kit; Metallothionein-II ELISA Kit; MT-II ELISA Kit
Abbreviation MT-2
Uniprot No. P04355
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat MT-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %80-95
1:2Average %95
Range %85-100
1:4Average %95
Range %86-102
1:8Average %90
Range %82-100
Recovery
The recovery of rat MT-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9585-105
EDTA plasma (n=4)9687-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.701 2.773 2.737 2.573
52.006 2.164 2.085 1.921
2.51.357 1.452 1.405 1.241
1.250.867 0.855 0.861 0.697
0.6250.563 0.562 0.563 0.399
0.3120.441 0.437 0.439 0.275
0.1560.283 0.297 0.290 0.126
00.165 0.163 0.164  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Gene References into Functions
  1. acute attack of experimental autoimmune encephalomyelitis was followed by increased expression of metallothioneins in molecular and granular cell layer of hippocampus. PMID: 27173051
  2. observe a huge increase in MT3 transcripts in both cortical and hippocampal areas, while the MT1/2 mRNA content increases slightly, returning to the levels measured in adolescent rats PMID: 27939232
  3. MT-I/II are required for the conditioning lesion effect and can promote axonal regeneration in the injured central nervous system. PMID: 25947372
  4. endogenous MT-I/II might play an important role in the pathogenesis of pain behaviors, participating in the initiation of inflammatory and neuropathic pain rather than in their maintenance PMID: 23726995
  5. Metallothionein-2 expression is regulated by psychological stress in adult male and female rats. PMID: 23291980
  6. Zinc pre-treatment improved MT-1 and MT-2 gene expressions in kidney which significantly alleviated acute toxicity of depleted uranium. PMID: 22703381
  7. The MT mRNA level displays isoform-specific changes: those of MT-1 do not change appreciably, whereas those of MT-2 increase significantly in 2-hr endotoxemia, with or without obstructive jaundice. PMID: 22100509
  8. Expression of MT2 in the adult rat testes and during development PMID: 20109269
  9. The solution structures of Cd(3)-thiolate cluster containing beta-domain of mouse beta-MT-1 and rat beta-MT-2 show high structural similarities, but widely differing structure dynamics. PMID: 19609577
  10. Results provide evidence for the heterogeneous distribution of MT(2) receptors in different parts and layers of the gastrointestinal tract, which could indicate a physiological role of melatonin in the gastrointestinal tract. PMID: 19004484
  11. TTR-MT1/2 complexes may be functionally significant not only in healthy conditions but also in Abeta deposition in Alzheimer disease. PMID: 18237193

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Protein Families Metallothionein superfamily, Type 1 family
Database Links

KEGG: rno:689415

STRING: 10116.ENSRNOP00000062585

UniGene: Rn.115549

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