Rat Mothers against decapentaplegic homolog 7,Smad7 ELISA Kit

Instructions
Code CSB-E09225r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name SMAD family member 7
Alternative Names Smad7 ELISA Kit; Madh7 ELISA Kit; Mothers against decapentaplegic homolog 7 ELISA Kit; MAD homolog 7 ELISA Kit; Mothers against DPP homolog 7 ELISA Kit; SMAD family member 7 ELISA Kit; SMAD 7 ELISA Kit; Smad7 ELISA Kit
Abbreviation SMAD7
Uniprot No. O88406
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Smad7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %84
Range %80-92
1:10Average %97
Range %91-105
1:20Average %105
Range %100-110
1:40Average %93
Range %86-98
Recovery
The recovery of rat Smad7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9288-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.760 2.652 2.706 2.522
5002.031 1.976 2.004 1.820
2501.358 1.304 1.331 1.147
1250.954 0.939 0.947 0.763
62.50.501 0.524 0.513 0.329
31.20.423 0.439 0.431 0.247
15.60.257 0.249 0.253 0.069
00.181 0.187 0.184
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Gene References into Functions
  1. Human umbilical cord mesenchymal stem cells exosomes may promote Smad7 expression by inhibiting miR125b5p to increase myocardial repair in the rat acute myocardial infarction model. PMID: 29484378
  2. The results demonstrated that miRNA-195 activated hepatic stellate cells (HSCs) by targeting Smad7. PMID: 28929107
  3. the present findings indicated that miR-15a inhibition protects cardiomyocytes against H/R-induced apoptosis by upregulating the expression of its target, SMAD7, and downregulating the NF-kappaB p65 subunit. PMID: 28440490
  4. miR-21 blocks norepinephrine-mediated rat granulosa cell apoptosis by targeting SMAD7. PMID: 28473352
  5. Smad7 influenced NF-kappaB expression by regulating reactive oxygen species generation, and induced glomerular mesangial cell apoptosis to counter the Ang II-promoted proliferation. PMID: 28988741
  6. Cell internal signal transduction, miR-187 regulating Smad7 expression, plays a vital role in retinal ganglion cell survival. PMID: 26463627
  7. Data suggest that post-surgical heterotopic ossification (HO) may be prevented in vivo by local delivery of mothers against decapentaplegic homolog 7 (SMAD7). PMID: 26807862
  8. Smad-7 was highly expressed in the degenerative discs. PMID: 25811233
  9. MicroRNA-92a inhibition attenuates hypoxia/reoxygenation-induced cardiomyocyte apoptosis by up-regulating Smad7 expression. PMID: 24941323
  10. miR-17-5p promotes hepatic stellate cell proliferation and activation, at least in part via reduction of Smad7 PMID: 25915722
  11. Study found that STRAP and Smad7 are significantly decreased in the pilocarpine-induced epileptic rats compared with the normal rats PMID: 24577865
  12. findings suggest that canonical TGFbeta signaling induces suture closure at least in part through activation of FGF and ERK signaling and that Smad7 plays an important role in maintaining suture patency PMID: 23959527
  13. Smad7 gene transfer inhibits fibrogenic responses of cornea in rats after photorefractive keratectomy PMID: 23595375
  14. Overexpression of Smad7 suppressed ROS/MMP9-dependent collagen synthesis through regulation of heme oxygenase-1. PMID: 23661026
  15. silencing the expression of the DNMT1 gene ameliorated the suppression of Smad7 expression by TGF-beta1 PMID: 24211420
  16. In the early stage of diabetic nephropathy, increase in BMP-7 and Smad6/7 expression may play a role in the feedback regulation and restrain the development of diabetic nephropathy. PMID: 17437042
  17. the ectopic expression of Smad7 suppresses the dI1 and dI3 neural fates and concomitantly increases the number of dI4-dI6 spinal neurons. PMID: 21718693
  18. Enalapril increased the expression of Smad7 in renal interstitial fibrosis in rats. PMID: 19197123
  19. Transforming growth factor beta 1 (TGF-beta1)inhibits expression of Smad7 during activation of pancreatic stellate cells. PMID: 20943464
  20. Letter: Report decreased expression of Smad7 in chronic pancreatitis. PMID: 20182310
  21. data imply that decreased inhibitory Smad 7 signal in cardiac fibroblasts may play a role in the pathogenesis of cardiac fibrosis in the postmyocardial infarct heart PMID: 11959632
  22. TGF-beta-induced apoptosis in mesangial cells is mediated through the activation of caspase-3 by Smad7, but not by Smad2 or Smad3. PMID: 12234288
  23. role in post-translational regulation of Muc4 via the transforming growth factor-beta and interferon-gamma pathways in rat mammary epithelial cells PMID: 12668667
  24. Smad7 has a role in renal fibrosis in association with hypertension PMID: 15743788
  25. YB-1 activation via the Jak1 signaling pathway is required and sufficient to confer interferon-gamma-dependent activation of the smad7 gene. PMID: 16278212
  26. Expressions of HGF and Smad7 were increased significantly in the kidneys of diabetic rats. PMID: 16297324
  27. Epigallocatechin gallate enhanced the expression of Smad7. PMID: 17541159
  28. Potassium supplementation can reduce renal inflammation and hence, could modulate the progression of kidney injury in chronic kidney disease through upregulation of renal Smad7. PMID: 17634402
  29. Smad7 overexpression suppresses peritoneal inflammation induced by peritoneal dialysis fluid. PMID: 17704451
  30. Blockade of the TGF-beta/Smad signaling pathway may represent a novel therapeutic approach to prevent peritoneal fibrosis in patients treated with peritoneal dialysis. PMID: 17855642
  31. induction of Smad7 by HGF serves to limit acquisition of the myofibroblast phenotype in alveolar epithelial cells PMID: 18988920

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Subcellular Location Nucleus, Cytoplasm
Protein Families Dwarfin/SMAD family
Tissue Specificity Ubiquitous.
Database Links

KEGG: rno:81516

STRING: 10116.ENSRNOP00000024831

UniGene: Rn.29980

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