Rat Muscarinic acetylcholine receptor M3(CHRM3) ELISA kit

Instructions
Code CSB-EL005383RA
Size 96T,5×96T,10×96T
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name cholinergic receptor, muscarinic 3
Alternative Names Chrm3 ELISA kit; Chrm-3Muscarinic acetylcholine receptor M3 ELISA kit
Abbreviation CHRM3
Uniprot No. P08483
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CHRM3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %80-88
1:2Average %89
Range %86-93
1:4Average %98
Range %95-101
1:8Average %93
Range %90-96
Recovery
The recovery of rat CHRM3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-96
EDTA plasma (n=4)9187-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.275 2.241 2.258 2.113
51.906 1.868 1.887 1.742
2.51.404 1.469 1.437 1.292
1.250.993 0.968 0.981 0.836
0.6250.594 0.567 0.581 0.436
0.3120.403 0.431 0.417 0.272
0.1560.307 0.327 0.317 0.172
00.144 0.146 0.145
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Gene References into Functions
  1. Data suggest that, in atrial myocardium (or isolated cardiomyocytes), activation of muscarinic receptors M3 (Chrm3) leads to shortening of action potentials via suppression of L-type calcium channels; the major potassium channels are not affected, but inwardly rectifying potassium channels are suppressed. PMID: 27858307
  2. M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product. PMID: 26626425
  3. Our results suggest that the mechanism for the deficits in learning and memory of rats with chronic fluorosis may be associated with the decreased expressions of M1 and M3 in mAChRs PMID: 25417050
  4. The expression of beta3-adrenoceptor and muscarinic type 3 receptor immuno-reactivity in the major pelvic ganglion of the rat. PMID: 25920933
  5. The ventricular myocardium of rat pups differs from that of mature rats by a far greater sensitivity to selective stimulation of M3 receptors, suggesting an important functional role of M3 receptors in the newborn rats, which declines with age. PMID: 26033578
  6. Neonatal hypoglycemia decreased total muscarinic receptors (p < 0.001) with reduced muscarinic M1, M2, and M3 receptor genes (p < 0.001) in one month old rats. PMID: 25474381
  7. Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis. PMID: 25681120
  8. Activation of muscarinic receptors leads to rapid activation of several kinases that regulates contraction of longitudinal muscle of colon. PMID: 25891767
  9. The relative mRNA expression of M3 and M5 mAChR in bones of a rat osteoporosis model, was investigated. PMID: 24866457
  10. The upregulation of M-mAChR during myocardial hypertrophy could relieve the hypertrophic response provoked by angiotensin II. PMID: 24028210
  11. Muscarinic M3 receptors are downregulated in the brain stem following spinal cord injury. PMID: 23184186
  12. These results suggest a complex antagonistic interplay between G(q)-activated PKC and Gbetagamma in regulation of L-VDCC, in which multiple cytosolic segments of alpha(1C) are involved. PMID: 22990911
  13. Muscarinic receptor expression is developmentally regulated; M1, M3, and M4 receptors are the main subtypes expressed in oligodendrocyte progenitor cells. PMID: 21913336
  14. Data suggest that Chrm3 is up-regulated in cell membranes of parotid glands in a model of periodontitis and participates in potassium release; Chrm3 expression appears to return to control level upon indomethacin treatment of periodontitis. PMID: 22513211
  15. M3 subtype of muscarinic acetylcholine receptor promotes cardioprotection via the suppression of miR-376b-5p PMID: 22396777
  16. Taurine supplementation of low-protein diet rats restored Ach-M3R, Synt1, SNAP-25 protein levels and increased sarcoendoplasmatic reticulum Ca2+-ATPase (SERCA) 3 protein expression in rats on a low-protein diet. PMID: 21543213
  17. NGF expression increased but M3 subtype muscarinic receptor expression decreased in the seminal vesicle of diabetic rats. PMID: 22141271
  18. activation of M3R dimers requires a conformational change of the N-terminal segment of the i3 loop PMID: 22031716
  19. structure of the G(q/11)-coupled M3 mAChR ('M3 receptor', from rat) bound to the bronchodilator drug tiotropium and identify the binding mode for this clinically important drug PMID: 22358844
  20. Our findings suggest an interaction between vitamin D(3) and muscarinic M3 receptors in regulating insulin secretion from pancreas. PMID: 20655720
  21. Expression of muscarinic receptor M1, M3, M5 subunits decreased in the flocculus after unilateral labyrinthectomy. PMID: 19160866
  22. study showed that M and M receptors, IP3 and cGMP were functionally regulated during diabetes as function of age PMID: 21054404
  23. Chrm3 receptor is located on presynaptic glutamatergic terminals afferent to the recorded pyramidal neuron, thus decreasing glutamate release. PMID: 20600670
  24. muscarinic M(1) receptor showed a significant decrease and muscarinic M(3) receptor subtype showed a significant increased binding in the cerebral cortex of hypoglycemic rats compared to diabetic and control PMID: 21126518
  25. We found a tendency in the gene expression of muscarinic receptor subtypes from M2 toward M3 after birth trauma. PMID: 20445960
  26. NF-kappaB signalling plays an essential role in controlling the expression of the muscarinic M(3) receptor. PMID: 20541544
  27. Acetylcholine through muscarinic M1 and M3 receptors stimulated calcium release from the pancreatic islets. PMID: 19554469
  28. Increased beta-catenin activity is associated with M(3) mAChR's binding during myocardial infarction. PMID: 19473345
  29. Results highlight long-term low dose somatotropin and insulin treatment in regulating muscarinic M1 and M3 cholinergic and NMAR1 and mGluR5 glutamate receptor subtypes in aging rats and rejuvenation of brain function. PMID: 19666081
  30. An in situ disulfide cross-linking strategy reveals the structure of the inactive and active state of the M3 muscarinic receptor by location of residues in transmembrane (TM) I close to Cys532, a residue present in the central portion of TM VII. PMID: 12056896
  31. in normal bladders both M(2) and M(3) receptors can induce contraction and in the denervated bladder, the M(2) and the M(3) receptors interact in a facilitatory manner to mediate contraction. PMID: 12185001
  32. The m1-m5 muscarinic subtypes mapped to the following chromosomes: Chrm1, chromosome 1; Chrm2, chromosome 4; Chrm3, chromosome 17; Chrm4, chromosome 3; and Chrm5, chromosome 3. PMID: 12433399
  33. The presence of M3-like receptors on mesencephalic GABAergic neurones was confirmed PMID: 14766941
  34. evidence for the presence of M(2)- and M(3)-mAChR, at the mRNA and protein level, in the rat myometrium and that estrogen induces an increase in myometrial responsiveness to mAChR agonists PMID: 15062561
  35. central muscarinic M1 and M3 receptor subtypes functional balance is suggested to regulate sympathetic and parasympathetic activity, which in turn control the islet cell proliferation and glucose homeostasis PMID: 15350825
  36. data suggest a conformational link between the highly conserved Asp-113, Arg-165, and Tyr-250 residues which is critical for receptor activation PMID: 15572356
  37. agonist activation of the M(3) muscarinic acetylcholine receptor results in pronounced conformational changes PMID: 15870064
  38. a conformational change occurs adjacent to the ligand-binding pocket of the M(3) muscarinic acetylcholine receptor after agonist binding PMID: 16093246
  39. spontaneous contractions in the neonatal rat bladder are enhanced by activation of M2 and M3 receptors PMID: 16709645
  40. Interaction of the third intracellular loop of SET protein with M3 muscarinic receptor (M3-MR) leads to reduced M3-MR signaling capacity. PMID: 17065150
  41. conclude that previously reported differences relate to the use of inhibitors rather than experimental protocols and that the overall data do not support a role for PLC in M(3) muscarinic receptor-mediated rat bladder contraction PMID: 17596535
  42. In reticular thalamic nucleus, the m3-immunolabeling was distributed in a large part of somata and in proximal dendrite shafts than in the distal dendrite region. PMID: 17845913
  43. Generation of an agonistic binding site for blockers of the M(3) muscarinic acetylcholine receptor. PMID: 18237275
  44. In the inflamed bladder, NO seems to be released via cholinergic stimuli through mucosal muscarinic M3/M5 receptors, presumably on urothelial cells, affecting bladder function PMID: 18246091
  45. The muscarinic receptors in the rat gastric mucosal segments were composed of M(1), M(2) and M(3) subtypes PMID: 18938154
  46. Taken together, these results suggest that muscarinic M3 receptor is involved in the regulation of glucose uptake and/or lipolysis in adipose tissue. PMID: 19111904
  47. Stimulation of the presinaptic M1-muscarinic receptors of the bladder cholinergic nerve terminals and M3-muscarinic receptors located on the bladder detrusor muscle induce the release of urinary bladder-derived relaxant factor. PMID: 19416629
  48. results demonstrate that activation of muscarinic receptors exerts diverse effects on GABAergic transmission in the entorhinal cortex PMID: 19494196
  49. The muscarinic receptors present in undifferentiated Fisher-rat thyroid epithelial cells belong to the M(3) subtype. Their binding affinities for various antagonists was determined. PMID: 19627722
  50. increased PKC-epsilon activity is associated with M3-mAChR during myocardial ischemia PMID: 19685039
  51. There is strong evidence for the functional significance of M(3) receptor expression in the vaginal muscularis. PMID: 19741053
  52. Immunoglobulins from scleroderma patients inhibit the muscarinic receptor activation in internal anal sphincter smooth muscle cells. PMID: 19779020

Show More

Hide All

Subcellular Location Cell membrane, Multi-pass membrane protein, Cell junction, synapse, postsynaptic cell membrane, Multi-pass membrane protein, Basolateral cell membrane, Multi-pass membrane protein
Protein Families G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM3 sub-subfamily
Database Links

KEGG: rno:24260

STRING: 10116.ENSRNOP00000067435

UniGene: Rn.87735

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved. 鄂ICP备15011166号-1