Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Code CSB-E07443r
Size 96T,5×96T,10×96T
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Product Details

Target Name
N-acetyl-β-D-glucosaminidase,NAG
Alternative Names
Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
Abbreviation
NAG
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range
1.56 mIU/mL-100 mIU/mL
Sensitivity
0.39 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-100
1:2Average %96
Range %91-102
1:4Average %87
Range %84-95
1:8Average %95
Range %92-101
Recovery
The recovery of rat NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10095-105
EDTA plasma (n=4)9690-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
1002.495 2.471 2.483 2.306
501.955 1.987 1.971 1.794
251.356 1.368 1.362 1.185
12.50.934 0.963 0.949 0.772
6.250.587 0.597 0.592 0.415
3.120.425 0.451 0.438 0.261
1.560.361 0.374 0.368 0.191
00.175 0.178 0.177  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat NAG ELISA Kit was designed for the quantitative measurement of Rat NAG protein in serum, plasma, cell culture supernates, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL .

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Target Background

Function
(From Uniprot)
Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
Subcellular Location
Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
Protein Families
Glycosyl hydrolase 20 family
Database Links

KEGG: rno:294673

UniGene: Rn.203067

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