Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Code CSB-E07443r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name N-acetyl-β-D-glucosaminidase,NAG
Alternative Names Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
Abbreviation NAG
Uniprot No. Q6AXR4
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 1.56 mIU/mL-100 mIU/mL
Sensitivity 0.39 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %95
Range %90-100
1:2Average %96
Range %91-102
1:4Average %87
Range %84-95
1:8Average %95
Range %92-101
The recovery of rat NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10095-105
EDTA plasma (n=4)9690-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
1002.495 2.471 2.483 2.306
501.955 1.987 1.971 1.794
251.356 1.368 1.362 1.185
12.50.934 0.963 0.949 0.772
6.250.587 0.597 0.592 0.415
3.120.425 0.451 0.438 0.261
1.560.361 0.374 0.368 0.191
00.175 0.178 0.177  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
Subcellular Location Lysosome
Protein Families Glycosyl hydrolase 20 family
Database Links

KEGG: rno:294673

UniGene: Rn.203067

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