Rat N-acetyl-D-glucosamine kinase(NAGK) ELISA kit

Code CSB-EL015415RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name N-acetylglucosamine kinase
Alternative Names Nagk ELISA Kit; N-acetyl-D-glucosamine kinase ELISA Kit; N-acetylglucosamine kinase ELISA Kit; EC ELISA Kit; GlcNAc kinase ELISA Kit
Abbreviation NAGK
Uniprot No. P81799
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat NAGK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-99  
1:2 Average % 87  
Range % 84-95  
1:4 Average % 93  
Range % 89-98  
1:8 Average % 95  
Range % 83-102  
The recovery of rat NAGK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 91-104  
EDTA plasma (n=4) 96 90-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 1.593 1.621 1.607 1.510  
500 1.302 1.359 1.331 1.234  
250 0.952 0.958 0.955 0.858  
125 0.637 0.654 0.646 0.549  
62.5 0.391 0.376 0.384 0.287  
31.2 0.265 0.270 0.268 0.171  
15.6 0.187 0.175 0.181 0.084  
0 0.095 0.098 0.097    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.
Gene References into Functions
  1. this work studied the role played by NAGK in axonal development. PMID: 26467288
  2. Data shows associations between NAGK, speckle, paraspeckle and general transcription factor suggesting its regulatory roles in gene expression. PMID: 25921606
  3. Upregulation of dendritic complexity is a non-canonical function of NAGK. PMID: 24625575
Protein Families Eukaryotic-type N-acetylglucosamine kinase family
Database Links

KEGG: rno:297393

STRING: 10116.ENSRNOP00000039247

UniGene: Rn.105985


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