Rat Noggin(NOG) ELISA kit

Instructions
Code CSB-EL015917RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name noggin
Alternative Names SYM1, SYNS1, symphalangism 1 (proximal)
Abbreviation NOG
Uniprot No. Q62809
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NOG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 90-100
1:2 Average % 90
Range % 85-94
1:4 Average % 85
Range % 80-89
1:8 Average % 98
Range % 94-102
Recovery
The recovery of rat NOG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 101-114
EDTA plasma (n=4) 87 82-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.712 2.583 2.648 2.447
5 2.105 2.040 2.073 1.872
2.5 1.310 1.362 1.336 1.135
1.25 0.783 0.815 0.799 0.598
0.625 0.479 0.451 0.465 0.264
0.312 0.341 0.327 0.334 0.133
0.156 0.265 0.279 0.272 0.071
0 0.197 0.204 0.201  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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Target Data

Function Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite (By similarity). Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (By similarity).
Subcellular Location Secreted
Protein Families Noggin family
Tissue Specificity Prominently expressed in the CNS. High levels found in mitral and tufted cells in the olfactory bulb, piriform cortex of the brain and Purkinje cells in the cerebellum. Low level expression seen in the lung, skeletal muscle and skin.
Database Links

STRING: 10116.ENSRNOP00000030212

UniGene: Rn.10154

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