Rat Noggin(NOG) ELISA kit

Code CSB-EL015917RA
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q62809
Lead Time 5-7 working days
Abbreviation NOG
Protein Biological Process 1 Developmental Protein
Target Name noggin
Alias SYM1, SYNS1, symphalangism 1 (proximal)
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.
HGNC 21535
RGD 620783
MGI 1916487
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NOG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 90-100
1:2 Average % 90
Range % 85-94
1:4 Average % 85
Range % 80-89
1:8 Average % 98
Range % 94-102
Recovery
The recovery of rat NOG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 101-114
EDTA plasma (n=4) 87 82-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.712 2.583 2.648 2.447
5 2.105 2.040 2.073 1.872
2.5 1.310 1.362 1.336 1.135
1.25 0.783 0.815 0.799 0.598
0.625 0.479 0.451 0.465 0.264
0.312 0.341 0.327 0.334 0.133
0.156 0.265 0.279 0.272 0.071
0 0.197 0.204 0.201  
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Function Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite (By similarity). Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (By similarity).
Subcellular Location Secreted
Protein Families Noggin family
Tissue Specificity Prominently expressed in the CNS. High levels found in mitral and tufted cells in the olfactory bulb, piriform cortex of the brain and Purkinje cells in the cerebellum. Low level expression seen in the lung, skeletal muscle and skin.
Database Links

STRING: 10116.ENSRNOP00000030212

UniGene: Rn.10154

Pathway TGF-beta signaling pathway

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