Rat Nuclear respiratory factor 2(NRF2) ELISA kit

Code CSB-EQ027869RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O54968
Lead Time 3-5 working days
Abbreviation NRF2
Target Name Nuclear respiratory factor 2(NRF2)
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 34.4 pg/mL-2200 pg/mL
Sensitivity 8.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NRF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 100
Range % 88-105
1:2 Average % 107
Range % 97-112
1:4 Average % 94
Range % 87-99
1:8 Average % 99
Range % 90-104
Recovery
The recovery of rat NRF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 84-97
EDTA plasma (n=4) 103 92-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2200 2.518 2.433 2.476 2.295
1100 1.972 1.927 1.950 1.769
550 1.245 1.285 1.265 1.084
275 0.833 0.804 0.819 0.638
137.5 0.504 0.516 0.510 0.329
68.75 0.346 0.340 0.343 0.162
34.4 0.267 0.251 0.259 0.078
0 0.178 0.184 0.181  
References
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Function Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (By similarity).
Subcellular Location Cytoplasm, cytosol, Nucleus
Protein Families BZIP family, CNC subfamily
Database Links

KEGG: rno:83619

STRING: 10116.ENSRNOP00000002114

UniGene: Rn.10867

Pathway Protein processing in endoplasmic reticulum
Cell ageing and metabolism

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