Rat Sialate O-acetylesterase(SIAE) ELISA kit

Instructions
Code CSB-EL021286RA
Size 96T,5×96T,10×96T
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Product Details

Target Name sialic acid acetylesterase
Alternative Names Siae ELISA kit; Ysg2Sialate O-acetylesterase ELISA kit; EC 3.1.1.53 ELISA kit; Sialic acid-specific 9-O-acetylesterase ELISA kit; Yolk sac protein 2) [Cleaved into: Sialate O-acetylesterase small subunit; Sialate O-acetylesterase large subunit] ELISA kit
Abbreviation SIAE
Uniprot No. P82450
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Tags & Cell Markers
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SIAE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 108
Range % 104-112
1:2 Average % 89
Range % 85-93
1:4 Average % 104
Range % 100-108
1:8 Average % 85
Range % 80-90
Recovery
The recovery of rat SIAE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 93-99
EDTA plasma (n=4) 89 84-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.741 2.704 2.723 2.632
500 2.282 2.105 2.194 2.103
250 1.273 1.304 1.289 1.198
125 0.609 0.631 0.620 0.529
62.5 0.329 0.334 0.332 0.241
31.25 0.201 0.199 0.200 0.109
15.6 0.136 0.134 0.135 0.044
0 0.090 0.092 0.091  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid.
Subcellular Location Lysosome
Tissue Specificity Widely expressed.
Database Links

STRING: 10116.ENSRNOP00000042202

UniGene: Rn.21775

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