Rat Superoxide dismutase [Mn], mitochondrial(SOD2) ELISA kit

Instructions
Code CSB-EL022398RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name superoxide dismutase 2, mitochondrial
Alternative Names Sod2 ELISA Kit; Superoxide dismutase [Mn] ELISA Kit; mitochondrial ELISA Kit; EC 1.15.1.1 ELISA Kit
Abbreviation SOD2
Uniprot No. P07895
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.87 pg/mL-120 pg/mL
Sensitivity 0.47 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SOD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %89
Range %84-92
1:2000Average %95
Range %91-98
1:4000Average %88
Range %84-91
1:8000Average %99
Range %93-102
Recovery
The recovery of rat SOD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-93
EDTA plasma (n=4)10198-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1202.427 2.398 2.413 2.307
601.887 1.862 1.875 1.769
301.449 1.402 1.426 1.320
150.803 0.813 0.808 0.702
7.50.492 0.466 0.479 0.373
3.750.292 0.287 0.290 0.184
1.870.202 0.213 0.208 0.102
00.107 0.105 0.106
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Citations

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Target Background

Function
(From Uniprot)
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Gene References into Functions
  1. The SIRT3-SOD2 signaling pathway is involved in mitochondrial dysfunction induced by hemorrhagic shock PMID: 27067422
  2. These benefits appear to be attributed to the enhanced interaction between STAT3 and MnSOD. These findings shed a light on a new role of STAT3 in oxidative stress and suggest that ZYZ451 is likely an effective cardio-protective agent. PMID: 26721595
  3. SOD2 mediates amifostine-induced protection in PC12 cells exposed to glutamate. PMID: 26770652
  4. Selective upregulation of SOD2 in the left ventricles of rat hearts during L-NAME induced heart failure. PMID: 25978844
  5. SOD2 might regulate neuroinflammation by controlling the microglial activities. PMID: 26231211
  6. Study demonstrated that knockdown of MnSOD in vitro leads to reactive oxygene species generation as well as induction of functional mitochondrial biogenesis. PMID: 24563852
  7. Data indicate a functionally active mitochondrial pool of p38beta mitogen activated protein kinase (p38beta MAPK), and that this kinase phosphorylates manganese superoxide dismutase (MnSOD) in an 17beta-estradiol (E2)-dependent manner. PMID: 24465521
  8. Data suggest that expression of mitochondrial MnSOD/Sod2 (and CuZnSOD/Sod1) is down-regulated as retinal neurons undergo apoptosis following onset of diabetes/diabetic retinopathy. PMID: 24527463
  9. miR-146a could down-regulate the SOD2 protein expression. PMID: 23935993
  10. Hyperbaric oxygen therapy alters SOD2 protein and mRNA levels after brain injury in a time-dependent manner. PMID: 23275324
  11. The present findings suggest that oxidative stress occurring in the brain of immature rats during and following the seizures induced by DL-HCA is apparently due to both the increased free radical production and the limited antioxidant defense. PMID: 23238024
  12. Down-regulation of Mn-SOD expression may play an important role in the aggravation of oxidative stress in endemic fluorosis. PMID: 23157833
  13. SOD2 protects cardiac progenitor cells from apoptosis following xanthine/xanthine oxidase treatment. PMID: 22758933
  14. There was no difference in the expression of Mn-SOD between OLETF rats and LETO rats in type 2 diabetes. PMID: 22981416
  15. Data suggest that local overexpression of MnSOD in rat skeletal muscle in vivo (by electroporation to transfer the human gene) is sufficient to protect muscle against insulin resistance caused by oxidative stress of high-fat diet. PMID: 22829583
  16. Only the chronic administration of higher doses of alpha-tocopherol is associated with the increased activity and expression of Mn-SOD in rats. PMID: 22732938
  17. The increased expression of antioxidant SOD2, specifically in hippocampal neurons, will provide protection from age-related cognitive decline. PMID: 21942371
  18. Berry diets did not alter COX-2 levels, but blueberry diet significantly reduced MnSOD levels in rats with reflux-induced esophageal adenocarcinoma. PMID: 22043833
  19. Changes in Mn-superoxide dismutase expression in rat hippocampus induced by single and triple exposure to moderate hypobaric hypoxia PMID: 22171425
  20. Upregulation of MnSOD in the aging heart was directly associated with attenuated levels of oxidative stress, including iNOS. PMID: 19297546
  21. Cigarette smoke aggravates reflux-induced BE and potentially accelerates the progression of BE to EAC through the loss of manganese superoxide dismutase (MnSOD), and overexpression of NF-kappaB- and COX-2-mediated factors. PMID: 21506880
  22. Increased H4K20me3 at sod2 contributes to its downregulation and is important in the development of diabetic retinopathy and in the metabolic memory phenomenon. PMID: 21357467
  23. Results suggest that the upregulation of TNFalpha, NFkappaB and MnSOD after deafferentation in the vestibular nuclei may constitute a favorable neuronal environment for the vestibular compensation process. PMID: 21143912
  24. Apoptosis of the retinal neurons in early diabetic rats may correlate with the decline of the activity and mRNA expression of MnSOD. PMID: 20654064
  25. PGC-1alpha is induced by cerebral ischemia leading to upregulation of UCP2 and SOD2, thereby providing a neuroprotective effect against ischemic brain injury in the hippocampus by ameliorating oxidative stress PMID: 19774674
  26. Chronic stress isolation results in mitochondrial dysfunction and MnSOD release into the cytosol. PMID: 19893080
  27. Data highlight the role played by some residues in ratSOD2 and suggest a fine regulation of the enzyme occurring in vivo. PMID: 19384983
  28. MnSod has a role in improving neuronal survival after hypoxia/ischemia PMID: 12577622
  29. Mn-SOD may play a role in regulating ROS and matrix degradation involving mechanical deterioration of the stress-deprived tendon tissue PMID: 15375579
  30. MnSOD is not affected by acute stressors PMID: 16238459
  31. analysis of transcriptional regulation of the superoxide dismutase-2 kappaB element in rat embryonic neurons and astrocytes PMID: 17023425
  32. Experimental abdominal aortic aneurysm formation is associated with early increases in MnSOD expression PMID: 17196988
  33. loss of MnSOD protein contributes to the reduced level of its enzymatic activity and plays a key role in the induction of esophagitis PMID: 17349848
  34. upregulated in neurogenic hypertension PMID: 19047792
  35. both the proportion of SOD2 immunopositive type 1 spiral ganglion cells and the intensity of immunoreactivity were elevated near cochlear apex PMID: 19376215
  36. overexpression in renal proximal tubular cells prevents hyperglycemia-induced changes PMID: 19439219

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Subcellular Location Mitochondrion matrix
Protein Families Iron/manganese superoxide dismutase family
Database Links

KEGG: rno:24787

STRING: 10116.ENSRNOP00000025794

UniGene: Rn.10488

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